Dataset Information

Acoustic frequency and optimum sonochemical production at single and multi-bubble scales: A modeling answer to the scaling dilemma.

ABSTRACT: The present work consists of an innovative approach aiming to address the scalability dilemma of the sonochemical activity dependency of acoustic frequency. The study originates from the discordance of observations between the theoretical investigations of the sonochemical activity of the single acoustic cavitation bubble in function of the acoustic frequency, in one hand, and the experimental findings regarding the optimal frequency condition, mainly in terms of pollutant degradation, in the other hand. A single bubble and an up-scaled model of the sonochemical activity are suggested and simulations were conducted based on both of them over the frequencies 20, 200, 300, 360, 443, 500, 600 and 800 kHz under an oxygen atmosphere. The results reveal that the sonochemical production at single bubble scale is monotonously decreasing with the increase of frequency, while all the products demonstrate an absolute optimum of sonochemical production at 200 kHz, except HO^• that attains its maximum molar yield under 300 kHz. Besides, the production of the predominant species, namely HO₂^•, HO^• and O₃, manifests a clear rebound at 500 kHz. All the present results were compared to and confirmed by experimental findings, while the scalability of the concentrations of sonochemically produced species was discussed using a parameter we introduced as "the mass focusing factor".

SUBMITTER: Kerboua K

PROVIDER: S-EPMC7786541 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Acoustic frequency and optimum sonochemical production at single and multi-bubble scales: A modeling answer to the scaling dilemma.

Kerboua Kaouther K Hamdaoui Oualid O Alghyamah Abdulaziz A

Ultrasonics sonochemistry 20200914

The present work consists of an innovative approach aiming to address the scalability dilemma of the sonochemical activity dependency of acoustic frequency. The study originates from the discordance of observations between the theoretical investigations of the sonochemical activity of the single acoustic cavitation bubble in function of the acoustic frequency, in one hand, and the experimental findings regarding the optimal frequency condition, mainly in terms of pollutant degradation, in the ot ...[more]

PMID: 32971392

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Project description:Sonoporation is the membrane disruption generated by ultrasound and has been exploited as a non-viral strategy for drug and gene delivery. Acoustic cavitation of microbubbles has been recognized to play an important role in sonoporation. However, due to the lack of adequate techniques for precise control of cavitation activities and real-time assessment of the resulting sub-micron process of sonoporation, limited knowledge has been available regarding the detail processes and correlation of cavitation with membrane disruption at the single cell level. In the current study, we developed a combined approach including optical, acoustical, and electrophysiological techniques to enable synchronized manipulation, imaging, and measurement of cavitation of single bubbles and the resulting cell membrane disruption in real-time. Using a self-focused femtosecond laser and high frequency ultrasound (7.44MHz) pulses, a single microbubble was generated and positioned at a desired distance from the membrane of a Xenopus oocyte. Cavitation of the bubble was achieved by applying a low frequency (1.5MHz) ultrasound pulse (duration 13.3 or 40?s) to induce bubble collapse. Disruption of the cell membrane was assessed by the increase in the transmembrane current (TMC) of the cell under voltage clamp. Simultaneous high-speed bright field imaging of cavitation and measurements of the TMC were obtained to correlate the ultrasound-generated bubble activities with the cell membrane poration. The change in membrane permeability was directly associated with the formation of a sub-micrometer pore from a local membrane rupture generated by bubble collapse or bubble compression depending on ultrasound amplitude and duration. The impact of the bubble collapse on membrane permeation decreased rapidly with increasing distance (D) between the bubble (diameter d) and the cell membrane. The effective range of cavitation impact on membrane poration was determined to be D/d=0.75. The maximum mean radius of the pores was estimated from the measured TMC to be 0.106±0.032?m (n=70) for acoustic pressure of 1.5MPa (duration 13.3?s), and increased to 0.171±0.030?m (n=125) for acoustic pressure of 1.7MPa and to 0.182±0.052?m (n=112) for a pulse duration of 40?s (1.5MPa). These results from controlled cell membrane permeation by cavitation of single bubbles revealed insights and key factors affecting sonoporation at the single cell level.

Dataset Information

Acoustic frequency and optimum sonochemical production at single and multi-bubble scales: A modeling answer to the scaling dilemma.

Publications

Acoustic frequency and optimum sonochemical production at single and multi-bubble scales: A modeling answer to the scaling dilemma.

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