Ontology highlight
ABSTRACT:
SUBMITTER: Sharma S
PROVIDER: S-EPMC7788559 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Sharma Sheetal S Anand Roopesh R Zhang Xuzhu X Francia Sofia S Michelini Flavia F Galbiati Alessandro A Williams Hannah H Ronato Daryl A DA Masson Jean-Yves JY Rothenberg Eli E Cejka Petr P d'Adda di Fagagna Fabrizio F
Cell reports 20210101 1
The MRE11-RAD50-NBS1 (MRN) complex supports the synthesis of damage-induced long non-coding RNA (dilncRNA) by RNA polymerase II (RNAPII) from DNA double-strand breaks (DSBs) by an unknown mechanism. Here, we show that recombinant human MRN and native RNAPII are sufficient to reconstitute a minimal functional transcriptional apparatus at DSBs. MRN recruits and stabilizes RNAPII at DSBs. Unexpectedly, transcription is promoted independently from MRN nuclease activities. Rather, transcription depen ...[more]