Dataset Information

Low arterial oxygen partial pressure induces pulmonary thrombocytopenia in patients and a mouse model.

ABSTRACT:

Background

Recent basic studies demonstrate that the lung is a primary organ of platelet biogenesis. However, whether the pathophysiological state of the lung affect the platelets is little known. We aim to investigate the incidence of thrombocytopenia in patients with pulmonary infection (PIN) and risk factors associated with pulmonary thrombocytopenia.

Methods

In total, 11,941 patients with pulmonary infection (PIN) were enrolled, and patients with other three infectious diseases were collected as controls. The incidence of thrombocytopenia was compared, and the risk factors associated with thrombocytopenia in PIN patients were investigated by multivariate analysis. To explore the mechanism of thrombocytopenia, hypoxic model was constructed. Blood platelet counts from the angular vein (PLTs), left ventricle (PLT_post) and right ventricle (PLT_pre) were determined. Megakaryocytes identified by anti-CD41 antibody were detected through flow cytometry and immunofluorescence.

Results

The incidence of thrombocytopenia in PIN was higher than that in other three infectious diseases (9.8% vs. 6.4%?~?5.0%, P?2) was an important risk factor for thrombocytopenia (OR?=?0.88; P?2 induced thrombocytopenia. The difference between the PLT_post and PLT_pre (?PLT_post-pre), representing the production of platelets in the lungs, was significantly attenuated in hypoxic mice when compared with normoxic mice (F?=?25.47, P?ConclusionThere is a high incidence for thrombocytopenia in PIN patients. Low PaO₂-induced thrombocytopenia is associated with impaired generation of platelet in the lungs.

SUBMITTER: Wu L

PROVIDER: S-EPMC7789170 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Low arterial oxygen partial pressure induces pulmonary thrombocytopenia in patients and a mouse model.

Wu Limeng L Guo Ninghong N Xu Zhenyan Z Wang Wei W Xiong Qinmei Q Hu Jinzhu J Wan Rong R Hong Kui K

BMC pulmonary medicine 20210106 1

<h4>Background</h4>Recent basic studies demonstrate that the lung is a primary organ of platelet biogenesis. However, whether the pathophysiological state of the lung affect the platelets is little known. We aim to investigate the incidence of thrombocytopenia in patients with pulmonary infection (PIN) and risk factors associated with pulmonary thrombocytopenia.<h4>Methods</h4>In total, 11,941 patients with pulmonary infection (PIN) were enrolled, and patients with other three infectious disease ...[more]

PMID: 33407285

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Project description:ObjectivesLongitudinal hemodynamic follow-up is important in the management of pulmonary hypertension (PH). This study aimed to evaluate the potential of MR 4-dimensional (4D) flow imaging to predict changes in the mean pulmonary arterial pressure (mPAP) during serial investigations.MethodsForty-four adult patients with PH or at risk of developing PH repeatedly underwent routine right heart catheterization (RHC) and near-term MR 4D flow imaging of the main pulmonary artery. The duration of vortical blood flow along the main pulmonary artery was evaluated from MR 4D velocity fields using prototype software and converted to an MR 4D flow imaging-based mPAP estimate (mPAPMR) by a previously established model. The relationship of differences between RHC-derived baseline and follow-up mPAP values (ΔmPAP) to corresponding differences in mPAPMR (ΔmPAPMR) was analyzed by means of regression and Bland-Altman analysis; the diagnostic performance of ΔmPAPMR in predicting mPAP increases or decreases was investigated by ROC analysis.ResultsAreas under the curve for the prediction of mPAP increases and decreases were 0.92 and 0.93, respectively. With the natural cutoff ΔmPAPMR = 0 mmHg, mPAP increases (decreases) were predicted with an accuracy, sensitivity, and specificity of 91% (91%), 85% (89%), and 94% (92%), respectively. For patients in whom 4D flow allowed a point estimate of mPAP (mPAP > 16 mmHg), ΔmPAPMR correlated strongly with ΔmPAP (r = 0.91) and estimated ΔmPAP bias-free with a standard deviation of 5.1 mmHg.ConclusionsMR 4D flow imaging allows accurate non-invasive prediction and quantification of mPAP changes in adult patients with PH or at risk of developing PH.Trial registrationClinicalTrials.gov identifier: NCT00575692 and NCT01725763 KEY POINTS: • MR 4D flow imaging allows accurate non-invasive prediction of mean pulmonary arterial pressure increases and decreases in adult patients with or at risk of developing pulmonary hypertension. • In adult patients with mean pulmonary arterial pressure > 16 mmHg, MR 4D flow imaging allows estimation of longitudinal mean pulmonary arterial pressure changes without bias with a standard deviation of 5.1 mmHg.

Dataset Information

Low arterial oxygen partial pressure induces pulmonary thrombocytopenia in patients and a mouse model.

Background

Methods

Results

Publications

Low arterial oxygen partial pressure induces pulmonary thrombocytopenia in patients and a mouse model.

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