Dataset Information

Hydrophobic insertion-based engineering of tumor cell-derived exosomes for SPECT/NIRF imaging of colon cancer.

ABSTRACT:

Background

Tumor cell-derived exosomes (TEx) have emerged as promising nanocarriers for drug delivery. Noninvasive multimodality imaging for tracing the in vivo trafficking of TEx may accelerate their clinical translation. In this study, we developed a TEx-based nanoprobe via hydrophobic insertion mechanism and evaluated its performance in dual single-photon emission computed tomography (SPECT) and near-infrared fluorescence (NIRF) imaging of colon cancer.

Results

TEx were successfully isolated from HCT116 supernatants, and their membrane vesicle structure was confirmed by TEM. The average hydrodynamic diameter and zeta potential of TEx were 110.87?±?4.61 nm and -9.20?±?0.41 mV, respectively. Confocal microscopy and flow cytometry findings confirmed the high tumor binding ability of TEx. The uptake rate of ^99mTc-TEx-Cy7 by HCT116 cells increased over time, reaching 14.07?±?1.31% at 6 h of co-incubation. NIRF and SPECT imaging indicated that the most appropriate imaging time was 18 h after the injection of ^99mTc-TEx-Cy7 when the tumor uptake (1.46%?±?0.06% ID/g) and tumor-to-muscle ratio (8.22?±?0.65) peaked. Compared with radiolabeled adipose stem cell derived exosomes (^99mTc-AEx-Cy7), ^99mTc-TEx-Cy7 exhibited a significantly higher tumor accumulation in tumor-bearing mice.

Conclusion

Hydrophobic insertion-based engineering of TEx may represent a promising approach to develop and label exosomes for use as nanoprobes in dual SPECT/NIRF imaging. Our findings confirmed that TEx has a higher tumor-targeting ability than AEx and highlight the potential usefulness of exosomes in biomedical applications.

SUBMITTER: Jing B

PROVIDER: S-EPMC7789573 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Hydrophobic insertion-based engineering of tumor cell-derived exosomes for SPECT/NIRF imaging of colon cancer.

Jing Boping B Gai Yongkang Y Qian Ruijie R Liu Zhen Z Zhu Ziyang Z Gao Yu Y Lan Xiaoli X An Rui R

Journal of nanobiotechnology 20210106 1

<h4>Background</h4>Tumor cell-derived exosomes (TEx) have emerged as promising nanocarriers for drug delivery. Noninvasive multimodality imaging for tracing the in vivo trafficking of TEx may accelerate their clinical translation. In this study, we developed a TEx-based nanoprobe via hydrophobic insertion mechanism and evaluated its performance in dual single-photon emission computed tomography (SPECT) and near-infrared fluorescence (NIRF) imaging of colon cancer.<h4>Results</h4>TEx were success ...[more]

PMID: 33407513

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Dataset Information

Hydrophobic insertion-based engineering of tumor cell-derived exosomes for SPECT/NIRF imaging of colon cancer.

Background

Results

Conclusion

Publications

Hydrophobic insertion-based engineering of tumor cell-derived exosomes for SPECT/NIRF imaging of colon cancer.

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