Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.

ABSTRACT:

Background

Metastasis accounts for ninety percent of breast cancer (BrCa) mortality. Cortactin, Ras homologous gene family member A (RhoA), and Rho-associated kinase (ROCK) raise cellular motility in favor of metastasis. Claudins (CLDN) belong to tight junction integrity and are dysregulated in BrCa. Thus far, epidemiologic evidence regarding the association of different pro-metastatic genes with pathological phenotypes of BrCa is largely inconsistent. This study aimed to determine the possible transcriptional models of pro-metastatic genes incorporate in holding the integrity of epithelial cell-cell junctions (CTTN, RhoA, ROCK, CLDN-1, CLDN-2, and CLDN-4), for the first time, in association with clinicopathological features of primary BrCa.

Methods

In a consecutive case-series design, 206 newly diagnosed non-metastatic eligible BrCa patients with histopathological confirmation (30-65 years) were recruited in Tabriz, Iran (2015-2017). Real-time RT-PCR was used. Then fold changes in the expression of target genes were measured.

Results

ROCK amplification was associated with the involvement of axillary lymph node metastasis (ALNM; OR_adj.?=?3.05, 95%CI 1.01-9.18). Consistently, inter-correlations of CTTN-ROCK (??=?0.226, P?+ BrCa. In addition, the overexpression of CLDN-4 was frequently observed in tumors identified by ALNM⁺ or grade III (P?adj.?=?1.96, 95%CI 1.02-3.77). ROCK was also expressed in luminal-B BrCa tumors (P?ConclusionsFor the first time, our findings suggested that the inter-correlations of CTTN-ROCK and RhoA-ROCK were significant transcriptional profiles determined in association with ALNM involvement; therefore the overexpression of ROCK may serve as a potential molecular marker for lymphatic metastasis. The provided binary transcriptional profiles need more approvals in different clinical features of BrCa metastasis.

SUBMITTER: Mazloomi SM 

PROVIDER: S-EPMC7789694 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature