Dataset Information

Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.

ABSTRACT:

Background

Metastasis accounts for ninety percent of breast cancer (BrCa) mortality. Cortactin, Ras homologous gene family member A (RhoA), and Rho-associated kinase (ROCK) raise cellular motility in favor of metastasis. Claudins (CLDN) belong to tight junction integrity and are dysregulated in BrCa. Thus far, epidemiologic evidence regarding the association of different pro-metastatic genes with pathological phenotypes of BrCa is largely inconsistent. This study aimed to determine the possible transcriptional models of pro-metastatic genes incorporate in holding the integrity of epithelial cell-cell junctions (CTTN, RhoA, ROCK, CLDN-1, CLDN-2, and CLDN-4), for the first time, in association with clinicopathological features of primary BrCa.

Methods

In a consecutive case-series design, 206 newly diagnosed non-metastatic eligible BrCa patients with histopathological confirmation (30-65 years) were recruited in Tabriz, Iran (2015-2017). Real-time RT-PCR was used. Then fold changes in the expression of target genes were measured.

Results

ROCK amplification was associated with the involvement of axillary lymph node metastasis (ALNM; OR_adj.?=?3.05, 95%CI 1.01-9.18). Consistently, inter-correlations of CTTN-ROCK (??=?0.226, P?+ BrCa. In addition, the overexpression of CLDN-4 was frequently observed in tumors identified by ALNM⁺ or grade III (P?adj.?=?1.96, 95%CI 1.02-3.77). ROCK was also expressed in luminal-B BrCa tumors (P?ConclusionsFor the first time, our findings suggested that the inter-correlations of CTTN-ROCK and RhoA-ROCK were significant transcriptional profiles determined in association with ALNM involvement; therefore the overexpression of ROCK may serve as a potential molecular marker for lymphatic metastasis. The provided binary transcriptional profiles need more approvals in different clinical features of BrCa metastasis.

SUBMITTER: Mazloomi SM

PROVIDER: S-EPMC7789694 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.

Mazloomi Seyed-Mohammad SM Foroutan-Ghaznavi Mitra M Montazeri Vahid V Tavoosidana Gholamreza G Fakhrjou Ashraf A Nozad-Charoudeh Hojjatollah H Pirouzpanah Saeed S

Cancer cell international 20210106 1

<h4>Background</h4>Metastasis accounts for ninety percent of breast cancer (BrCa) mortality. Cortactin, Ras homologous gene family member A (RhoA), and Rho-associated kinase (ROCK) raise cellular motility in favor of metastasis. Claudins (CLDN) belong to tight junction integrity and are dysregulated in BrCa. Thus far, epidemiologic evidence regarding the association of different pro-metastatic genes with pathological phenotypes of BrCa is largely inconsistent. This study aimed to determine the p ...[more]

PMID: 33407452

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Project description:BACKGROUND:Per1, Per2, Per3, Cry1, Cry2, Bmal1, Npas2 and CLOCK genes are the eight core circadian clock genes. Low expression of these circadian clock genes plays an important role in the progression of cancers. However, its clinicopathological and prognostic value in patients with cancers remains controversial and inconclusive. We performed a meta-analysis of studies assessing the clinicopathological and prognostic significance of low expression of these genes in cancers. METHODS:Relevant studies were searched from the Cochrane Central Register of Controlled Trials, Embase, EBSCO, Ovid, PubMed, Science Direct, Wiley Online Library database, CNKI and Wan Fang database. The meta-analysis was performed by using STATA version 12 software. A random-effect model was employed to evaluate all pooled hazard ratios (HRs) and odd ratios (ORs). RESULTS:A total of 36 studies comprising 7476 cases met the inclusion criteria. Meta-analysis suggested that low expression of Per1 was associated with poor differentiation (Per1: OR=2.30, 95%CI: 1.36?3.87, P=0.002) and deeper invasion depth (Per1: OR=2.12, 95%CI: 1.62?2.77, ?<0.001); low Per2 expression was correlated with poor differentiation (Per2: OR=2.41, 95%CI: 1.53?3.79, ?<0.001), worse TNM stage (Per2:OR=3.47, 95%CI: 1.88?6.42, P<0.001) and further metastasis (Per2:OR=2.35, 95%CI: 1.35?4.11, ?=0.003). Furthermore, the results revealed that low expressions of Per1 and Per2 were also correlated with poor overall survival of cancers (Per1: HR=1.35, 95%CI: 1.06?1.72, P=0.014; Per2: HR=1.43, 95%CI: 1.10?1.85, P=0.007). Subgroup analysis indicated that low Per1 and Per2 expressions were especially associated with poor prognosis of gastrointestinal caners (Per1: HR=1.33, 95%CI: 1.14?1.55, ?<0.001, ?2=4.2%; Per2: HR=1.62, 95%CI: 1.25?2.18, P<0.001, I2=0.0%). CONCLUSIONS:Our study suggested that low Per1, Per2 and Npas2 expression played a distinct and crucial role in progression of cancers. Low expressions of Per1 and Per2 could serve as unfavorable indicators for cancers prognosis, especially for gastrointestinal cancers.

Dataset Information

Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.

Background

Methods

Results

Publications

Profiling the expression of pro-metastatic genes in association with the clinicopathological features of primary breast cancer.

Similar Datasets

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