Dataset Information

Ultrastructural alteration of pulmonary tissue under conditions of high oxygen concentration.

ABSTRACT:

Objective

To determine the structure of pulmonary tissue under conditions of high oxygen concentration.

Methods

Ten-week-old C57BL male mice and control mice were exposed to 100% oxygen and to room air for 72 hours, respectively. To follow the progression of lesions, the mice were sacrificed at 6, 12, 24, 48, and 72 hours after 100% oxygen administration. Lung specimens obtained from these mice underwent morphologic analysis and immunofluorescence studies. We used scanning and transmission electron microscopy to determine the ultrastructure of the pulmonary capillaries, including the endothelial glycocalyx. To visualize the endothelial glycocalyx, we performed lanthanum nitrate staining.

Results

The survival rate of the 100% oxygen administration group was 5% (2/40) and that of the control group was 100%. Perivascular cavity enlargement was detected 12 hours after 100% oxygen administration and expanded over time. Ultrastructural analysis using electron microscopy revealed collapsed alveoli and pulmonary capillary wall and alveolar wall thickening in the 100% oxygen group. The pulmonary capillary endothelial glycocalyx was injured in the 100% oxygen group. The perivascular cavity decreased in mice that were returned to room air after 48 hours of 100% oxygen administration.

Conclusion

High-concentration oxygen causes perivascular cavity enlargement; this is thought to be a special characteristic of high oxygen damage. In addition, high-concentration oxygen may be involved in pulmonary endothelial glycocalyx injury.

SUBMITTER: Yano H

PROVIDER: S-EPMC7791376 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Ultrastructural alteration of pulmonary tissue under conditions of high oxygen concentration.

Yano Hirohisa H Kuroda Ayumi A Okada Hideshi H Tomita Hiroyuki H Suzuki Kodai K Takada Chihiro C Fukuda Hirotsugu H Kawasaki Yuki Y Muraki Isamu I Wakayama Yugo Y Kano Soichiro S Tamaoki Yuto Y Nishio Ayane A Inagawa Risa R Sampei So S Kamidani Ryo R Kakino Yoshinori Y Yasuda Ryu R Kitagawa Yuichiro Y Fukuta Tetsuya T Miyake Takahito T Kanda Norihide N Miyazaki Nagisa N Doi Tomoaki T Yoshida Takahiro T Suzuki Akio A Yoshida Shozo S Ogura Shinji S

International journal of clinical and experimental pathology 20201201 12

<h4>Objective</h4>To determine the structure of pulmonary tissue under conditions of high oxygen concentration.<h4>Methods</h4>Ten-week-old C57BL male mice and control mice were exposed to 100% oxygen and to room air for 72 hours, respectively. To follow the progression of lesions, the mice were sacrificed at 6, 12, 24, 48, and 72 hours after 100% oxygen administration. Lung specimens obtained from these mice underwent morphologic analysis and immunofluorescence studies. We used scanning and tra ...[more]

PMID: 33425101

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Project description:The present study uses electron microscopy to document ultrastructural characteristics of hippocampal GABAergic inhibitory synapses under resting and stimulated conditions in three experimental systems. Synaptic profiles were sampled from stratum pyramidale and radiatum of the CA1 region from (1) perfusion fixed mouse brains, (2) immersion fixed rat organotypic slice cultures, and from (3) rat dissociated hippocampal cultures of mixed cell types. Synapses were stimulated in the brain by a 5 min delay in perfusion fixation to trigger an ischemia-like excitatory condition, and by treating the two culture systems with 90 mM high K+ for 2-3 min to depolarize the neurons. Upon such stimulation conditions, the presynaptic terminals of the inhibitory synapses exhibited similar structural changes to those seen in glutamatergic excitatory synapses, with depletion of synaptic vesicles, increase of clathrin-coated vesicles and appearance of synaptic spinules. However, in contrast to excitatory synapses, no structural differences were detected in the postsynaptic compartment of the inhibitory synapses upon stimulation. There were no changes in the appearance of material associated with the postsynaptic membrane or the length and curvature of the membrane. Also no change was detected in the labeling density of gephyrin, a GABAergic synaptic marker, lining the postsynaptic membrane. Furthermore, virtually all inhibitory synaptic clefts remained rigidly apposed, unlike in the case of excitatory synapses where ~ 20-30% of cleft edges were open upon stimulation, presumably to facilitate the clearance of neurotransmitters from the cleft. The fact that no open clefts were induced in inhibitory synapses upon stimulation suggests that inhibitory input may not need to be toned down under these conditions. On the other hand, similar to excitatory synapse, EGTA (a calcium chelator) induced open clefts in ~ 18% of inhibitory synaptic cleft edges, presumably disrupting similar calcium-dependent trans-synaptic bridges in both types of synapses.

Dataset Information

Ultrastructural alteration of pulmonary tissue under conditions of high oxygen concentration.

Objective

Methods

Results

Conclusion

Publications

Ultrastructural alteration of pulmonary tissue under conditions of high oxygen concentration.

Similar Datasets

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