Ontology highlight
ABSTRACT:
SUBMITTER: Shishodia S
PROVIDER: S-EPMC7796277 | biostudies-literature | 2020 Dec
REPOSITORIES: biostudies-literature
Shishodia Shifali S Schofield Christopher J CJ
Molecules (Basel, Switzerland) 20201231 1
<i>N</i><sup>6</sup>-methyladenosine(m<sup>6</sup>A) is the most abundant modification in mRNA. Studies on proteins that introduce and bind m<sup>6</sup>A require the efficient synthesis of oligonucleotides containing m<sup>6</sup>A. We report an improved five-step synthesis of the m<sup>6</sup>A phosphoramidite starting from inosine, utilising a 1-<i>H</i>-benzotriazol-1-yloxytris(dimethylamino)phosphoniumhexafluorophosphate (BOP)<sub>-</sub>mediated S<sub>N</sub>Ar reaction in the key step. Th ...[more]