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A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.

ABSTRACT: FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence background.

SUBMITTER: Sheng W 

PROVIDER: S-EPMC7796701 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.

Sheng Wei W   Nick Setare Tahmasebi ST   Santos Elizabeth M EM   Ding Xinliang X   Zhang Jun J   Vasileiou Chrysoula C   Geiger James H JH   Borhan Babak B  

Angewandte Chemie (International ed. in English) 20181112 49

FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence backgroun  ...[more]

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