A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.
Ontology highlight
ABSTRACT:
SUBMITTER: Sheng W
PROVIDER: S-EPMC7796701 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Publications
A Near-Infrared Photoswitchable Protein-Fluorophore Tag for No-Wash Live Cell Imaging.
Sheng Wei W Nick Setare Tahmasebi ST Santos Elizabeth M EM Ding Xinliang X Zhang Jun J Vasileiou Chrysoula C Geiger James H JH Borhan Babak B
Angewandte Chemie (International ed. in English) 20181112 49
FR-1V, a fluorene-based aldehydic chromophore, binds its target protein as an imine to yield a highly bathochromic pigment, CF-2, a prototypic protein-dye tagging system whose NIR emission can be spatiotemporally switched ON by rapid UV-light activation. This is achieved through photoisomerization of the imine and its subsequent protonation. We demonstrate a no-wash protocol for live cell imaging of subcellular compartments in a variety of mammalian cell lines with minimal fluorescence backgroun ...[more]
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