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Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae).


ABSTRACT: The complete mitochondrial genome of the European blackfly, Simulium variegatum Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Chironomus tepperi Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367?bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene arrangement. The base composition of the genome is A (37.6%), T (35.3%), C (15.8%) and G (11.3%). The control region between 12S rRNA and tRNAIle is composed of 362?bp with no obvious repetitive motifs.

SUBMITTER: Day JC 

PROVIDER: S-EPMC7800089 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Sequence and organization of the complete mitochondrial genome of the blackfly <i>Simulium variegatum</i> (Diptera: Simuliidae).

Day John C JC   Gweon Hyun S HS   Post Rory J RJ  

Mitochondrial DNA. Part B, Resources 20161122 1


The complete mitochondrial genome of the European blackfly, <i>Simulium variegatum</i> Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of <i>Chironomus tepperi</i> Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene  ...[more]

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