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Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A₂ mating type.

ABSTRACT: Complete mitochondrial genome of Phytophthora infestans, A₂ mating type (MT) with a size of ?37,767?bp was sequenced. A total of 53 protein-coding genes are predicted on both strands, including 25 tRNA, 2 rRNA, and 18 respiratory proteins. Gene order of A₂MT was consistent with that established in A₁, despite high level of polymorphism in both coding and non-coding regions. The mtDNA of A₂MT was found to have 99.5% and 99.4% homology with Ia and Ib, whereas 94.7% and 94.3% with IIa and IIb, respectively. Study of repeats revealed a dinucleotide (AT)₉ specific to A₁ and homology of cox1 gene sequence revealed the relationship among 50 Phytophthora species.

SUBMITTER: Patil VU

PROVIDER: S-EPMC7800244 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type.

Patil Virupaksh U VU Vanishree G G Pattanayak Debasis D Sharma Sanjeev S Bhardwaj Vinay V Singh B P BP Chakrabarti S K SK

Mitochondrial DNA. Part B, Resources 20170216 1

Complete mitochondrial genome of Phytophthora infestans, A2 mating type (MT) with a size of ≅37,767 bp was sequenced. A total of 53 protein-coding genes are predicted on both strands, including 25 tRNA, 2 rRNA, and 18 respiratory proteins. Gene order of A2MT was consistent with that established in A1, despite high level of polymorphism in both coding and non-coding regions. The mtDNA of A2MT was found to have 99.5% and 99.4% homolo ...[more]

PMID: 33473726

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Correction to: Patil et al., Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type

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Project description:Phytophthora infestans is an oomycete that causes severe damage to potato, and is well known for its ability to evolve rapidly in order to overcome resistant potato varieties. An RNA silencing strategy was evaluated here to clarify if small interfering RNA homologous to selected genes in P. infestans could be targeted from the plant host to reduce the magnitude of the infection. As a proof-of-concept, a hairpin RNA (hp-RNA) construct using the GFP marker gene was designed and introduced in potato. At 72 hpi, a 55-fold reduction of the signal intensity of a corresponding GFP expressing P. infestans strain on leaf samples of transgenic plants, compared with wild-type potato, was detected. This suggests that an RNA interference construct in the potato host could be processed and target a transcript of the pathogen. Three genes important in the infection process of P. infestans, PiGPB1, PiCESA2, and PiPEC, together with PiGAPDH taking part in basic cell maintenance were subsequently tested using an analogous transgenic strategy. Out of these gene candidates, the hp-PiGPB1 targeting the G protein ?-subunit (PiGPB1) important for pathogenicity resulted in most restricted disease progress. Further, Illumina sequencing of inoculated transgenic potato leaves revealed sRNAs of 24/25 nt size homologous to the PiGPB1 gene in the transgenic plants indicating post-transcriptional silencing of the target gene. The work demonstrates that a host-induced gene-silencing approach is functional against P. infestans but is highly dependent on target gene for a successful outcome. This finding broadens the arsenal of control strategies to this important plant disease.

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