Dataset Information

Characterization of the complete chloroplast genome of the Eastern gamagrass, Tripsacum dactyloides.

ABSTRACT: Tripsacum dactyloides, known as eastern gamagrass, is used as a donor of valuable traits. It grows naturally in the same region where maize is commercially cultured in the USA and has the ability to hybridize to maize. The wild genotype of eastern gamagrass is threaten by the gene flow from the transgenic maize. The circular genome is 141,050?bp in length and contains 120 genes, including 73 protein-coding genes (PCG), 39 transfer RNA genes (tRNA) and eight ribosomal RNA genes (rRNA). The overall nucleotide composition is: 30.8% A, 19.2% C, 19.3% G, 30.7% T, with a total G?+?C content of 38.5%. The phylogenetic tree was constructed to explore the taxonomic status of Tripsacum dactyloides, which contributes to phylogenetic studies and further conservation strategies for this species.

SUBMITTER: Wang Y

PROVIDER: S-EPMC7800761 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Publications

Characterization of the complete chloroplast genome of the Eastern gamagrass, <i>Tripsacum dactyloides</i>.

Wang Yanfang Y Zhao Mingzhu M Li Li L Wang Kangyu K

Mitochondrial DNA. Part B, Resources 20171207 2

<i>Tripsacum dactyloides</i>, known as eastern gamagrass, is used as a donor of valuable traits. It grows naturally in the same region where maize is commercially cultured in the USA and has the ability to hybridize to maize. The wild genotype of eastern gamagrass is threaten by the gene flow from the transgenic maize. The circular genome is 141,050 bp in length and contains 120 genes, including 73 protein-coding genes (PCG), 39 transfer RNA genes (tRNA) and eight ribosomal RNA genes (rRNA). The ...[more]

PMID: 33474032

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Project description:Eastern gamagrass (Tripsacum dactyloides L.) belongs to the same tribe of the Poaceae family as maize (Zea mays L.) and grows naturally in the same region where maize is commercially produced in the USA. Although no evidence exists of gene flow from maize to eastern gamagrass in nature, experimental crosses between the two species were produced using specific techniques. As part of environmental risk assessment, the possibility of transgene flow from maize to eastern gamagrass populations in nature was evaluated with the objectives: (1) to assess the seeds of eastern gamagrass populations naturally growing near commercial maize fields for the presence of a transgenic glyphosate-tolerance gene (cp4 epsps) that would indicate cross-pollination between the two species, and (2) to evaluate the possibility of interspecific hybridization between transgenic maize used as male parent and eastern gamagrass used as female parent. A total of 46,643 seeds from 54 eastern gamagrass populations collected in proximity of maize fields in Illinois, USA were planted in a field in 2014 and 2015. Emerged seedlings were treated with glyphosate herbicide and assessed for survival. An additional 48,000 seeds from the same 54 eastern gamagrass populations were tested for the presence of the cp4 epsps transgene markers using TaqMan® PCR method. The results from these trials showed that no seedlings survived the herbicide treatment and no seed indicated presence of the herbicide tolerant cp4 epsps transgene, even though these eastern gamagrass populations were exposed to glyphosate-tolerant maize pollen for years. Furthermore, no interspecific hybrid seeds were produced from 135 hand-pollination attempts involving 1529 eastern gamagrass spikelets exposed to maize pollen. Together, these results indicate that there is no evidence of gene flow from maize to eastern gamagrass in natural habitats. The outcome of this study should be taken in consideration when assessing for environmental risks regarding the consequence of gene flow from transgenic maize to its wild relatives.

Dataset Information

Characterization of the complete chloroplast genome of the Eastern gamagrass, Tripsacum dactyloides.

Publications

Characterization of the complete chloroplast genome of the Eastern gamagrass, <i>Tripsacum dactyloides</i>.

Similar Datasets

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