Dataset Information

The complete mitochondrial genome of the hybrid Cyprinus capio Furong. [Cyprinus carpio L. mirror (?)×Cyprinus Carpio var. singuonensis (?)].

ABSTRACT: The complete mitochondrial genome of the hybrid of Cyprinus carpio L. mirror (?)×?Cyprinus Carpio var. singuonensis (?) was characterized first in this study. The total length of the genome was identical to the female parent as 16,581?bp, and the overall base composition was 31.80% A, 24.85% T, 27.55% C and 15.80% G, with a slight A?+?T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light-strand replication). This study discovered the 99.70% sequence identity between the hybrid and its female parent, which confirmed the maternal inheritance pattern followed by the mitochondrial genome of the hybrid. However, the sequence alignment of mitochondrial genomes between the hybrid and its female parent revealed a total of 47 variable sites in 15 genes or regions, especially 25 sense mutations in 6 PCGs. The complete mitochondrial genome sequence of the hybrid Cyprinus capio Furong may provide an important dataset for further study in mitochondrial inheritance mechanism.

SUBMITTER: Wang J

PROVIDER: S-EPMC7800765 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The complete mitochondrial genome of the hybrid Cyprinus capio Furong. [Cyprinus carpio L. mirror (♀)×Cyprinus Carpio var. singuonensis (♂)].

Wang Jinlong J Zou Li L Jiang Guomin G Zeng Ming M Li Chuanwu C Wu Yuanan Y Wang Dongwu D Liu Mingqiu M Liu Li L

Mitochondrial DNA. Part B, Resources 20160622 1

The complete mitochondrial genome of the hybrid of Cyprinus carpio L. mirror (♀)× Cyprinus Carpio var. singuonensis (♂) was characterized first in this study. The total length of the genome was identical to the female parent as 16,581 bp, and the overall base composition was 31.80% A, 24.85% T, 27.55% C and 15.80% G, with a slight A + T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region a ...[more]

PMID: 33473507

Similar Datasets

Project description:Perfluorooctane sulfonate (PFOS) has been manufactured for over 50 years in increasing quantities and has been used for several industrial and commercial aims. Due to the persistence and the bioaccumulation of this pollutant, it can be found worldwide in wildlife and humans. Biochemical effects of PFOS exposure are mainly studied in mammalian model species and information about effects on fish species remain largely scarce. This lack of toxicity data points out that there is an urgent need for the mechanistic molecular understanding of the mode of action of this pollutant. In the present study, common carp (Cyprinus carpio) was exposed through water for 14 days at concentrations of 0.1; 0.5 and 1 mg/l PFOS. Liver was selected as target tissue. Custom microarrays were constructed from cDNA libraries obtained with Suppression Subtractive Hybridization-Polymerase chain reaction (SSH-PCR) experiments. Microarray data revealed that the expression of several genes in the liver was influenced by PFOS exposure and real-time PCR was used to confirm these gene expression changes. The affected genes were mainly involved in energy metabolism, reproduction and stress response. Furthermore, the relative condition factor and the hepatosomatic index of the exposed fish were significantly lower after 14 days of exposure as well as the available glycogen reserves. At all levels of biological organization, indications of a trade-off between the metabolic cost of toxicant exposure on one hand and processes vital to the survival of the organism on the other hand were seen. Our results support the prediction that increases in energy expenditure negatively affects processes vital to the survival of an organism, such as growth. Keywords: PFOS, common carp, microarray, condition factor, energy reserves, metabolic cost There were 3 biological replicates for each exposure concentration. For each biological replicate control versus exposed hybridizations were carried out. The mean of the biological replicates was calculated for the differentially expressed genes.

Dataset Information

The complete mitochondrial genome of the hybrid Cyprinus capio Furong. [Cyprinus carpio L. mirror (?)×Cyprinus Carpio var. singuonensis (?)].

Publications

The complete mitochondrial genome of the hybrid <i>Cyprinus capio Furong. [Cyprinus carpio L. mirror</i> (♀)×<i>Cyprinus Carpio var. singuonensis</i> (♂)].

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets