Dataset Information

The complete mitochondrial genome of the hybrid Furong-crucian [Cyprinus capio Furong (?) × Carassius auratus red var (?)].

ABSTRACT: The complete mitochondrial genome of the hybrid of Cyprinus capio Furong (?)?×?Carassius auratus red var. (?) was characterized first in this study. The total length of the mitochondrial genome of Furong-crucian was identical to the female parent as 16,581?bp, and the overall base composition was 31.87% A, 24.81% T, 27.56% C and 15.76% G, with a slight A?+?T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light-strand replication). This study discovered the 99.3% sequence identity between the hybrid and its female parent, which confirmed the maternal inheritance pattern followed by the mitochondrial genome of the hybrid. However, the sequence alignment of mitochondrial genomes between the hybrid and its female parent revealed a total of 109 variable sites in 18 genes or regions, especially 32 sense mutations in 9 PCGs. The complete mitochondrial genome sequence of this hybrid Furong-crucian may provide an important dataset for further study in mitochondrial inheritance mechanism.

SUBMITTER: Zou L

PROVIDER: S-EPMC7800864 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome of the hybrid Furong-crucian [Cyprinus capio Furong (♀) × Carassius auratus red var (♂)].

Zou Li L Wang Jinlong J Zeng Ming M Liu Mingqiu M Wang Dongwu D Wu Yuanan Y Li Chuanwu C Jiang Guomin G Liu Li L

Mitochondrial DNA. Part B, Resources 20160712 1

The complete mitochondrial genome of the hybrid of Cyprinus capio Furong (♀) × Carassius auratus red var. (♂) was characterized first in this study. The total length of the mitochondrial genome of Furong-crucian was identical to the female parent as 16,581 bp, and the overall base composition was 31.87% A, 24.81% T, 27.56% C and 15.76% G, with a slight A + T bias. It contained 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 main non-coding ...[more]

PMID: 33473536

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Project description:BackgroundMelanocortin-3 and -4 receptors (MC3R and MC4R), G protein-coupled receptors, play vital roles in the regulation of energy homeostasis. To understand the functions of mc3r and mc4r in the energy homeostasis of red crucian carp (Carassius auratus red var., RCC), we cloned mc3r and mc4r, analyzed the tissue expression and localization of the genes, and investigated the effects of knockout of mc3r (mc3r +/-) and mc4r (mc4r +/-) in RCC.ResultsThe full-length cDNAs of RCC mc3r and mc4r were 1459 base pairs (bp) and 1894 bp, respectively. qRT-PCR indicated that mc3r and mc4r were profusely expressed in the brain, but lower expressed in the periphery tissues. ISH revealed that mc3r and mc4r were located in NPP, NPO, NAPv, NSC, NAT, NRL, NLTl, and NLTp of the brain, suggesting that mc3r and mc4r might regulate many physiological and behavioral aspects in RCC. To further verify the roles of mc3r and mc4r in energy homeostasis, the mc3r+/- and mc4r+/- fish were obtained by the CRISPR/Cas9 system. The average body weights, total lengths, body depths, and food intake of mc4r+/- fish were significantly higher than those of mc3r+/- and the normal wild-type (WT) fish, but there was no difference between the mc3r+/- and WT fish, indicating that the RCC phenotype and food intake were mainly influenced by mc4r but not mc3r. Interestingly, mc4r+/- fish displayed more visceral fat mass than mc3r+/- and WT fish, and mc3r+/- fish also exhibited slightly more visceral fat mass compared to WT. RNA-seq of the liver and muscle revealed that a large number of differentially expressed genes (DEGs) differed in WT vs. mc3r+/-, WT vs. mc4r+/-, and mc3r+/- vs. mc4r+/-, mainly related to lipid, glucose, and energy metabolism. The KEGG enrichment analysis revealed that DEGs were mainly enriched in pathways such as steroid biosynthesis, fatty acid metabolism, fatty acid biosynthesis, glycolysis/gluconeogenesis, wnt signaling pathway, PPAR signaling pathway, and MAPK signaling pathway, thereby affecting lipid accumulation and growth.ConclusionIn conclusion, these results will assist in the further investigation of the molecular mechanisms in which MC3R and MC4R were involved in the regulation of energy homeostasis in fish.

Dataset Information

The complete mitochondrial genome of the hybrid Furong-crucian [Cyprinus capio Furong (?) × Carassius auratus red var (?)].

Publications

The complete mitochondrial genome of the hybrid <i>Furong-crucian</i> [<i>Cyprinus capio Furong</i> (♀) × <i>Carassius auratus red var</i> (♂)].

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