Engineering of a bona fide light-operated calcium channel.
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ABSTRACT:
SUBMITTER: He L
PROVIDER: S-EPMC7801460 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Publications
Engineering of a bona fide light-operated calcium channel.
He Lian L Wang Liuqing L Zeng Hongxiang H Tan Peng P Ma Guolin G Zheng Sisi S Li Yaxin Y Sun Lin L Dou Fei F Siwko Stefan S Huang Yun Y Wang Youjun Y Zhou Yubin Y
Nature communications 20210111 1
The current optogenetic toolkit lacks a robust single-component Ca<sup>2+</sup>-selective ion channel tailored for remote control of Ca<sup>2+</sup> signaling in mammals. Existing tools are either derived from engineered channelrhodopsin variants without strict Ca<sup>2+</sup> selectivity or based on the stromal interaction molecule 1 (STIM1) that might crosstalk with other targets. Here, we describe the design of a light-operated Ca<sup>2+</sup> channel (designated LOCa) by inserting a plant-de ...[more]
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