Dataset Information

(¹³C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

ABSTRACT:

Background & aims

Non-alcoholic fatty liver disease (NAFLD) is characterised by the presence of hepatic steatosis in the absence of other causes of secondary hepatic fat accumulation, and is usually associated with visceral, metabolically active obesity. However, the subclinical effects of body and liver fat accumulation on liver function are still unclear.

Methods

We used orally administered (¹³C)-methacetin and breath test to quantify the efficiency of hepatic extraction from portal blood flow and liver microsomal function in 81 participants, in relation to presence/absence of ultrasonographic NAFLD, extent of body fat accumulation, insulin resistance, dietary models, and lifestyle.

Results

NAFLD was present in 23% of participants with normal weight, and prevalence increased with body fat and insulin resistance. Fat accumulation, NAFLD, and insulin resistance were associated with decreased hepatic extraction efficiency, and liver microsomal function was impaired in moderate-to-severe NAFLD. Caloric intake, dietary models, and lifestyles had a minor role in promoting functional changes.

Conclusions

The interplay between body fat accumulation, insulin resistance, and NAFLD is linked with altered hepatic extraction efficiency from blood flow and deranged microsomal function. Non-invasive diagnosis of subclinical alterations of liver function is relevant for primary and secondary prevention measures. Furthermore, the occurrence of NAFLD in lean individuals and the evidence that caloric intake, dietary models, and lifestyle played a minor role require further studies exploring the role of environmental factors in the natural history of these diseases.

Lay summary

Obesity is progressively increasing worldwide and is paralleled by fat accumulation in the liver (non-alcoholic fatty liver disease [NAFLD]), the most common chronic liver disease worldwide. NAFLD can alter liver structure and function, with a variety of consequences ranging from asymptomatic and subclinical alterations to cirrhosis and cancer. (¹³C)-Methacetin breath test, a non-invasive diagnostic tool, can reveal early subclinical alterations of liver dynamic function in individuals with obesity and in patients with NAFLD.

SUBMITTER: Molina-Molina E

PROVIDER: S-EPMC7806794 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Publications

(<sup>13</sup>C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

Molina-Molina Emilio E Shanmugam Harshitha H Di Ciaula Agostino A Grattagliano Ignazio I Di Palo Domenica Maria DM Palmieri Vincenzo O VO Portincasa Piero P

JHEP reports : innovation in hepatology 20201104 1

<h4>Background & aims</h4>Non-alcoholic fatty liver disease (NAFLD) is characterised by the presence of hepatic steatosis in the absence of other causes of secondary hepatic fat accumulation, and is usually associated with visceral, metabolically active obesity. However, the subclinical effects of body and liver fat accumulation on liver function are still unclear.<h4>Methods</h4>We used orally administered (<sup>13</sup>C)-methacetin and breath test to quantify the efficiency of hepatic extract ...[more]

PMID: 33490935

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Project description:Objectives: Fontan-associated liver disease (FALD) is the most common end-organ dysfunction affecting up to 70-80% of the Fontan population. The clinical significance of FALD is incompletely understood and no unambiguous correlation between hepatic function and FALD severity has been established. In this study, we sought to evaluate maximal liver function capacity with liver maximum function capacity test (LiMAx®) in adult Fontan patients. Methods: Thirty-nine adult Fontan patients (median age: 29.4 years [IQR 23.4; 37.4], median follow-up after Fontan operation: 23.9 years [IQR 17.8;26.4]) were analyzed in a cross-sectional observational study using LiMAx® test (Humedics GmbH, Berlin, Germany), laboratory testing, transient elastography (TE) and hepatic ultrasound. The LiMAx® test is based on the metabolism of 13C-methacetin, which is administered intravenously and cleaved by the hepatic cytochrome P4501A2 to paracetamol and 13CO2, which is measured in exhaled air and correlates with maximal liver function capacity. Results: Maximal liver function capacity assessed by LiMAx® test was normal in 28 patients (>315 μg/h*kg) and mildly to moderately impaired in 11 patients (140-314 μg/h*kg), while no patient displayed severe hepatic impairment (<139 μg/kg*h). No correlation was found between maximal liver function capacity and hepatic stiffness by TE (r 2 = -0.151; p = 0.388) or the presence of sonographic abnormalities associated with FALD (r 2 = -0.204, p = 0.24). There was, however, an association between maximal liver function capacity and the laboratory parameters bilirubin (r 2 = -0.333, p = 0.009) and γ-glutamyl transferase (r 2 = -0.367; p = 0.021). No correlation was detected between maximal liver function capacity and the severity of FALD (r 2 = -0.235; p = 0.152). Conclusion: To the best of our knowledge, this is the first study to evaluate maximal liver function capacity using LiMAx® test in Fontan patients, which is a useful complementary diagnostic instrument to assess chronic hepatic injury. Maximal liver function capacity was preserved in most of our adult Fontan patients despite morphologic evidence of FALD. Moreover, maximal liver function capacity does not correlate with the extent of FALD severity evaluated by sonography or laboratory analysis. Thus, the development and progression of FALD in Fontan patients is not a uniform process and diagnostics of chronic hepatic injury during follow-up should encompass various modalities.

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Dataset Information

(¹³C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

Background & aims

Methods

Results

Conclusions

Lay summary

Publications

(<sup>13</sup>C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

(13C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

Background & aims

Methods

Results

Conclusions

Lay summary

Publications

(<sup>13</sup>C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

(¹³C)-Methacetin breath test provides evidence of subclinical liver dysfunction linked to fat storage but not lifestyle.