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Using enantioselective indicator displacement assays to determine the enantiomeric excess of alpha-amino acids.

ABSTRACT: Enantioselective indicator displacement assays (eIDAs) were used for the determination of enantiomeric excess (ee) of alpha-amino acids as an alternative to the labor-intensive technique of chromatography. In this study, eIDAs were implemented by the use of two chiral receptors [(Cu(II)(1)](2+), [Cu(II)(2)](2+)) in conjunction with the indicator chrome azurol S. The two receptors were able to enantioselectively discriminate 13 of the 17 analyzed alpha-amino acids. Enantiomeric excess calibration curves were made using both receptors and then used to analyze true test samples to check the system's ability to determine ee accurately. The proposed method uses a conventional UV-vis spectrophotometer to monitor the colorimetric signal, which allows for a potential high-throughput screening (HTS) method for determining ee. The techniques created consistently produced results accurate enough for rapid preliminary determination of ee.

SUBMITTER: Leung D 

PROVIDER: S-EPMC7811882 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Using enantioselective indicator displacement assays to determine the enantiomeric excess of alpha-amino acids.

Leung Diana D   Folmer-Andersen J Frantz JF   Lynch Vincent M VM   Anslyn Eric V EV  

Journal of the American Chemical Society 20080821 37

Enantioselective indicator displacement assays (eIDAs) were used for the determination of enantiomeric excess (ee) of alpha-amino acids as an alternative to the labor-intensive technique of chromatography. In this study, eIDAs were implemented by the use of two chiral receptors [(Cu(II)(1)](2+), [Cu(II)(2)](2+)) in conjunction with the indicator chrome azurol S. The two receptors were able to enantioselectively discriminate 13 of the 17 analyzed alpha-amino acids. Enantiomeric excess calibration  ...[more]

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