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Computational approach for the identification of putative allergens from Cucurbitaceae family members.

ABSTRACT: Certain substances referred to as allergens, induce hypersensitivity (allergic reactions) which normally are considered to be innocuous, are small in size and incite IgE response. This study was focused to predict the putative allergens from other Cucurbitaceae family members using computational approach by analyzing the already reported allergens of the same family. The four reported allergens Cuc m 1, Cuc m 2, Cuc m 3 and Citr I 2 of Cucurbitaceae family were obtained from International Union of Immunological Societies, in which three were from Cucumis melo (Muskmelon) and one from Citrullus lanatus (Watermelon) respectively. BlastP analysis reported 44 similar sequences to these allergens from other members of Cucurbitaceae family namely Cucurbita moschata, Cucurbita pepo and Cucurbita maxima. The allergenicity of these sequences was predicted using AlgPred tool in which it revealed 26 protein sequences as putative allergens. These selected sequences were further analyzed for their physicochemical properties using ProtParam tool in which 13 sequences were found to satisfy the required parameters, and therefore further analyzed by AllerMatch™ and AllergenOnline tools to check the Codex Alimentarius rules for allergens. Finally, 13 sequences that were selected were structurally analyzed for similarity using PROMALS3D tool and phylogenetic relationship was established with the reported allergens using MEGA-X software. It was concluded that 13 sequences from Cucurbitaceae family belonging to different species of Pumpkin showed potential allergenicity based on the computational analysis that possibly can play a role in allergies and cross reactivity.

SUBMITTER: Neeharika D

PROVIDER: S-EPMC7813966 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundActinobacillus suis disease has been reported in a wide range of vertebrate species, but is most commonly found in swine. A. suis is a commensal of the tonsils of the soft palate of swine, but in the presence of unknown stimuli it can invade the bloodstream, causing septicaemia and sequelae such as meningitis, arthritis, and death. It is genotypically and phenotypically similar to A. pleuropneumoniae, the causative agent of pleuropneumonia, and to other members of the family Pasteurellaceae that colonise tonsils. At present, very little is known about the genes involved in attachment, colonisation, and invasion by A. suis (or related members of the tonsil microbiota).ResultsBioinformatic analyses of the A. suis H91-0380 genome were done using BASys and blastx in GenBank. Forty-seven putative adhesin-associated genes predicted to encode 24 putative adhesins were discovered. Among these are 6 autotransporters, 25 fimbriae-associated genes (encoding 3 adhesins), 12 outer membrane proteins, and 4 additional genes (encoding 3 adhesins). With the exception of 2 autotransporter-encoding genes (aidA and ycgV), both with described roles in virulence in other species, all of the putative adhesin-associated genes had homologues in A. pleuropneumoniae. However, the majority of the closest homologues of the A. suis adhesins are found in A. ureae and A. capsulatus--species not known to infect swine, but both of which can cause systemic infections.ConclusionsA. suis and A. pleuropneumoniae share many of the same putative adhesins, suggesting that the different diseases, tissue tropism, and host range of these pathogens are due to subtle genetic differences, or perhaps differential expression of virulence factors during infection. However, many of the putative adhesins of A. suis share even greater homology with those of other pathogens within the family Pasteurellaceae. Similar to A. suis, these pathogens (A. capsulatus and A. ureae) cause systemic infections and it is tempting to speculate that they employ similar strategies to invade the host, but more work is needed before that assertion can be made. This work begins to examine adhesin-associated factors that allow some members of the family Pasteurellaceae to invade the bloodstream while others cause a more localised infection.

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Computational approach for the identification of putative allergens from Cucurbitaceae family members.

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