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Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions.

ABSTRACT: Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water-oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D₂O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C₁₀E₅) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism.

SUBMITTER: Engelskirchen S

PROVIDER: S-EPMC7814304 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions.

Engelskirchen Sandra S Wellert Stefan S Holderer Olaf O Frielinghaus Henrich H Laupheimer Michaela M Richter Sven S Nestl Bettina B Nebel Bernd B Hauer Bernhard B

Frontiers in chemistry 20210105

Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water-oil interface. In the pr ...[more]

PMID: 33469526

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Project description:The hydrophobic ionic liquid [C8mim][PF6] (1-octyl-3-methylimidazolium hexafluorophosphate)-based bicontinuous microemulsion stabilized by the anionic surfactant [C4mim][AOT] (1-butyl-3-methylimidazolium bis(2-ethylhexyl) sulfosuccinate) was first tried as a medium for horseradish peroxidase (HRP)-triggered oxidative polymerization of aniline. The effects of the mass ratio of [C8mim][PF6]-to-water (α), the mass fraction of [C4mim][AOT] in the total mixture (γ), and temperature (T) on the enzymatic polymerization were investigated using UV-vis-NIR absorption, electron spin resonance, and small-angle X-ray scattering spectroscopy techniques. The bicontinuous microemulsion is demonstrated to play a template role in the biosynthesis of polyaniline (PANI). The conductivity of the resulting PANI depends on the microemulsion microstructure and the microstructure- and T-dependent catalytic properties of the solubilized HRP. With the increase in α, the conductivity of the synthesized PANI decreases due to the increase in the template curvature (decrease of the microdomain size) and the decrease in the activity and stability of HRP. Compared with α, γ has little effect on the microdomain size of the template; so, the γ-dependent change in the conductivity of PANI is mainly caused by the changes of the microstructure-dependent activity and stability of HRP. Over the range of 20-35 °C, T has little effect on the microdomain size, but it greatly changes the activity and stability of HRP. With the increase in T, the activity of HRP increases steadily, but its stability decreases significantly, which should be one of the reasons why the conductivity of PANI decreases with increasing T. In conclusion, lower values of α, γ, and T are favorable for the biosynthesis of conductive PANI. The present study not only deepens the insight into the role of the template in the process of PANI synthesis, but also opens up a green new way for the biosynthesis of the conducting polymer.

Dataset Information

Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions.

Publications

Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions.

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