Exploring the Diversity of Cysteine-Rich Natural Product Peptides via MS/MS Fingerprint Ions.

ABSTRACT: Natural product extracts present inherently complex matrices in which the identification of novel bioactive peptide species is challenged by low-abundance masses and significant structural and sequence diversity. Additionally, discovery efforts often result in the re-identification of known compounds, where modifications derived in vivo or during sample handling may obscure true sequence identity. Herein, we identify mass spectral (MS²) "fingerprint" ions characteristic of cyclotides, a diverse and biologically active family of botanical cysteine-rich peptides, based on regions of high sequence homology. We couple mass shift analysis with MS² spectral fingerprint ions cross referenced with CyBase-a cyclotide database-to discern unique mass species in Viola c

SUBMITTER: Parsley NC 

PROVIDER: S-EPMC7816094 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature