Dataset Information

A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.

ABSTRACT:

Introduction

Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings.

Methods

All experiments were performed on a mobile UV/VIS spectrophotometer. The levofloxacin concentration was quantified by using the amplitude of the second-order spectrum between 300 and 400?nm of seven calibrators. The concentration of spiked samples was calculated from the spectrum amplitude using linear regression. The method was validated for selectivity, specificity, linearity, accuracy and precision. Drugs frequently co-administered were tested for interference.

Results

The calibration curve was linear over a range of 2.5-50.0?mg/L for levofloxacin, with a correlation coefficient of 0.997. Calculated accuracy ranged from -5.2% to 2.4%. Overall precision ranged from 2.1% to 16.1%. Application of the Savitsky-Golay method reduced the effect of interferents on the quantitation of levofloxacin. Although rifampicin and pyrazinamide showed analytical interference at the lower limit of quantitation of levofloxacin concentrations, this interference had no implication on decisions regarding the levofloxacin dose.

Conclusions

A simple UV/VIS spectrophotometric method to quantify levofloxacin in saliva using a mobile nanophotometer has been validated. This method can be evaluated in programmatic settings to identify patients with low levofloxacin drug exposure to trigger personalized dose adjustment.

SUBMITTER: Alffenaar JC

PROVIDER: S-EPMC7816168 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.

Alffenaar Jan-Willem C JC Jongedijk Erwin M EM van Winkel Claudia A J CAJ Sariko Margaretha M Heysell Scott K SK Mpagama Stellah S Touw Daan J DJ

The Journal of antimicrobial chemotherapy 20210101 2

<h4>Introduction</h4>Therapeutic drug monitoring (TDM) for personalized dosing of fluoroquinolones has been recommended to optimize efficacy and reduce acquired drug resistance in the treatment of MDR TB. Therefore, the aim of this study was to develop a simple, low-cost, robust assay for TDM using mobile UV/visible light (UV/VIS) spectrophotometry to quantify levofloxacin in human saliva at the point of care for TB endemic settings.<h4>Methods</h4>All experiments were performed on a mobile UV/V ...[more]

PMID: 33089322

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Project description:Graphitic carbon nitride (g-C₃N₄) and titanium dioxide (TiO₂) were chosen as a model system to investigate photocatalytic abilities of heterojunction system under UV and visible light conditions. The use of g-C₃N₄ has been shown to be effective in the reduction in recombination through the interaction between the two interfaces of TiO₂ and g-C₃N₄. A simple method of preparing g-C₃N₄ through the pyrolysis of melamine was employed, which was then added to undoped TiO₂ material to form the g-C₃N₄-TiO₂ system. These materials were then fully characterized by X-ray diffraction (XRD), Brunauer Emmett Teller (BET), and various spectroscopic techniques including Raman, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), diffuse absorbance, and photoluminescence analysis. Photocatalysis studies were conducted using the model dye, rhodamine 6G utilizing visible and UV light irradiation. Raman spectroscopy confirmed that a composite of the materials was formed as opposed to a mixture of the two. Using XPS analysis, a shift in the nitrogen peak to that indicative of substitutional nitrogen was detected for all doped samples. This is then mirrored in the diffuse absorbance results, which show a clear decrease in band gap values for these samples, showing the effective band gap alteration achieved through this preparation process. When g-C₃N₄-TiO₂ samples were analyzed under visible light irradiation, no significant improvement was observed compared that of pure TiO₂. However, under UV light irradiation conditions, the photocatalytic ability of the doped samples exhibited an increased reactivity when compared to the undoped TiO₂ (0.130 min-1), with 4% g-C₃N₄-TiO₂ (0.187 min-1), showing a 43.9% increase in reactivity. Further doping to 8% g-C₃N₄-TiO₂ lead to a decrease in reactivity against rhodamine 6G. BET analysis determined that the surface area of the 4% and 8% g-C₃N₄-TiO₂ samples were very similar, with values of 29.4 and 28.5 m²/g, respectively, suggesting that the actual surface area is not a contributing factor. This could be due to an overloading of the system with covering of the active sites resulting in a lower reaction rate. XPS analysis showed that surface hydroxyl radicals and oxygen vacancies are not being formed throughout this preparation. Therefore, it can be suggested that the increased photocatalytic reaction rates are due to successful interfacial interactions with the g-C₃N₄-doped TiO₂ systems.

Dataset Information

A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.

Introduction

Methods

Results

Conclusions

Publications

A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva.

Similar Datasets

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