Ontology highlight
ABSTRACT: Background
A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its pathogenicity, one-day-old goslings were inoculated separately with GoAstV via oral and subcutaneous injection routes.Results
Clinical symptoms, gross and microscopic lesions, blood biochemical parameters and viral loads were detected and recorded for 20?days after infection. Typical gout was observed in experimental goslings. GoAstV can be replicated in tissues and cause pathological damage, especially in the kidney, liver, heart and spleen. Virus-specific genomic RNA was detected in blood, cloacal swabs and all representative tissues, and virus shedding was detected up to 20?days after inoculation, suggesting that GoAstV has a wide tissue tropism and spread systematically after inoculation. The viral copy numbers examined in kidney were the highest, followed by spleen and liver.Conclusion
This experiment determined the accurate value of viral loads and biochemical indicators of GoAstV-induced goslings. These findings increase our understanding of the pathogenicity of GoAstV in goslings and provide more reference for future research.
SUBMITTER: Yin D
PROVIDER: S-EPMC7818743 | biostudies-literature | 2021 Jan
REPOSITORIES: biostudies-literature
Yin Dan D Tian Jiajun J Yang Jing J Tang Yi Y Diao Youxiang Y
BMC veterinary research 20210120 1
<h4>Background</h4>A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its pathogenicity, one-day-old goslings were inoculated separately with GoAstV via oral and subcutaneous injection routes.<h4>Results</h4>Clinical symptoms, gross and microscopic lesions, blood biochem ...[more]