Ontology highlight
ABSTRACT:
SUBMITTER: Yan X
PROVIDER: S-EPMC7821101 | biostudies-literature | 2021 Feb
REPOSITORIES: biostudies-literature
The Journal of cell biology 20210201 2
CRISPR (clustered regularly interspaced short palindromic repeats)-based gene inactivation provides a powerful means for linking genes to particular cellular phenotypes. CRISPR-based screening typically uses large genomic pools of single guide RNAs (sgRNAs). However, this approach is limited to phenotypes that can be enriched by chemical selection or FACS sorting. Here, we developed a microscopy-based approach, which we name optical enrichment, to select cells displaying a particular CRISPR-indu ...[more]