Dataset Information

Dopamine D1 receptor in the NAc shell is involved in delayed emergence from isoflurane anesthesia in aged mice.

ABSTRACT:

Background

Delayed emergence after general anesthesia tends to occur in the elderly population, but the mechanism remains unclear. Apart from age-related pharmacokinetic changes, the aging-induced structural and functional alterations in the arousal-promoting neural substrates should be considered. The nucleus accumbens (NAc) is a crucial arousal-related nucleus, in which activating medium spiny neurons (MSNs) expressing dopamine D1 receptor (D1R) could facilitate the arousal from natural sleep. Meanwhile, the dopaminergic systems decline with aging in multiple brain regions. However, whether the age-related decline in D1R in the NAc shell attenuates its arousal-promoting capacity from general anesthesia remains to be elucidated.

Methods

We first verified the delayed emergence from isoflurane anesthesia and examined the corresponding changes of electroencephalogram (EEG) power in aged mice. In turn, the arousal-modulating capacity of D1R was characterized in the young and aged cohorts by microinjection of D1R agonist/antagonist into the NAc shell. Furthermore, to address the possible mechanism responsible for the attenuated arousal-modulating capacity of the aged NAc, the expression of D1R in the NAc shell was measured and compared between young and aged mice.

Results

Our data indicated that compared with young mice, the emergence time in aged mice was notably longer, while EEG power in ? band (1-4Hz) was significantly higher and power in ? band (12-25Hz) was lower. Activating or inhibiting D1R in the NAc shell by microinjection D1R agonist/antagonist promoted or delayed the emergence process in young mice. Nevertheless, this modulation capacity of D1R in the NAc shell declined in aged mice, respectively. Meanwhile, downregulation of D1R expression in the NAc shell was detected in the aged brain.

Conclusion

Together, these results suggest that aging attenuates the arousal-modulating capacity of D1R in the NAc shell probably through downregulation of D1R expression therein, which may provide a potential explanation and a therapeutic target for increased sensitivity to anesthetics in the elderly patients.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC7821614 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Dopamine D1 receptor in the NAc shell is involved in delayed emergence from isoflurane anesthesia in aged mice.

Zhang Yi Y Gui Huan H Hu Lang L Li Chengxi C Zhang Jie J Liang Xiaoli X

Brain and behavior 20201022 1

<h4>Background</h4>Delayed emergence after general anesthesia tends to occur in the elderly population, but the mechanism remains unclear. Apart from age-related pharmacokinetic changes, the aging-induced structural and functional alterations in the arousal-promoting neural substrates should be considered. The nucleus accumbens (NAc) is a crucial arousal-related nucleus, in which activating medium spiny neurons (MSNs) expressing dopamine D1 receptor (D1R) could facilitate the arousal from natura ...[more]

PMID: 33094567

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Dataset Information

Dopamine D1 receptor in the NAc shell is involved in delayed emergence from isoflurane anesthesia in aged mice.

Background

Methods

Results

Conclusion

Publications

Dopamine D1 receptor in the NAc shell is involved in delayed emergence from isoflurane anesthesia in aged mice.

Similar Datasets

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