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Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of Lambs.

ABSTRACT: Our objective was to investigate the effect of methionine restriction and resuming supply on liver antioxidant response in lambs. The concentrations of methionine and its metabolites and the expression of the nuclear factor erythroid 2-related factor 2 (Nrf₂), a redox sensitive factor, were detected after methionine restriction treatment for 50 days and methionine supply recovery for 29 days. The expression of glutathione (GSH) S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were characterized at the level of transcription and translation. Methionine restriction can directly change the content of methionine and its metabolites in plasma and liver, and affect the redox state of lambs by activating the Nrf₂ signaling pathway. Liver tissue can adapt to oxidative environment by upregulating the expression of antioxidant enzymes such as GSH-Px and SOD. Moreover, it was found that there was a lag effect in the recovery of metabolism after methionine supplementation.

SUBMITTER: Liu R

PROVIDER: S-EPMC7822206 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of Lambs.

Liu Rong R Diao Qiyu Q Cui Kai K

Animals : an open access journal from MDPI 20201223 1

Our objective was to investigate the effect of methionine restriction and resuming supply on liver antioxidant response in lambs. The concentrations of methionine and its metabolites and the expression of the nuclear factor erythroid 2-related factor 2 (Nrf<sub>2</sub>), a redox sensitive factor, were detected after methionine restriction treatment for 50 days and methionine supply recovery for 29 days. The expression of glutathione (GSH) S-transferase (GST), superoxide dismutase (SOD), catalase ...[more]

PMID: 33374518

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Project description:The uptake and metabolism of methionine (Met) are critical for epigenetic regulation, redox homeostasis, and embryo development. Our previous study showed that appropriate supplementation of dietary Met promoted the birth weight and placental angiogenesis of high-prolific sows. To further explore the metabolic effect of Met on pregnant sows, we have evaluated the influence of dietary Met level on Met metabolism, and the relationship between metabolites of Met and reproductive performance, antioxidant ability, and placental angiogenesis throughout the gestation of high-prolific sows. Sixty sows (the 3rd parity, Large White) were randomly divided into 5 groups that were fed diets with standardized ileal digestible (SID) methionine-to-lysine (Met:Lys) ratios of 0.27 (control), 0.32, 0.37, 0.42, and 0.47 from the mating day (gestational d 0, G0d) until the farrowing day. HPLC-MS/MS analysis was used to simultaneously evaluate the metabolites related to Met, e.g. S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), homocysteine (Hcy), cysteine (Cys), and glutathione (GSH). The concentration of SAM and SAH in plasma had significant fluctuations, especially in late pregnancy. Increasing dietary Met supplementation significantly improved the plasma SAM and methylation potential (SAM-to-SAH ratio) at d 114 of pregnancy (G114d). Moreover, a positive association of the plasma SAM concentration at G114d was observed with the litter weight of born alive (P < 0.05; R 2 = 0.58). Furthermore, Hcy concentration in plasma was at the lowest level for 0.37 ratio group at G114d. However, it significantly increased during late pregnancy. Moreover, there were negative correlations between plasma Hcy concentration at G114d (P < 0.05) and the placental vascular density in the fold and stroma (P < 0.05). Compared with the control group, the expression of vascular endothelial growth factor-A (VEGF-A) in the placenta tissue of 0.37 ratio group increased significantly (P < 0.05). Collectively, these findings indicate that dietary Met:Lys ratio (0.37 to 0.57) in the pregnant diet dose not influence the antioxidant ability of the high-prolific sows; however, the improvement of fetal development and placental angiogenesis of high-prolific sows by supplementation of Met are closely associated to the key Met-related metabolite of SAM and Hcy, respectively.

Dataset Information

Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of Lambs.

Publications

Effect of Dietary Methionine Deficiency Followed by a Re-Feeding Phase on the Hepatic Antioxidant Activities of Lambs.

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