Dataset Information

Comprehensive Analysis of ceRNA Regulation Network Involved in the Development of Coronary Artery Disease.

ABSTRACT:

Background

Coronary artery disease (CAD) is one of the most common causes of sudden death with high morbidity in recent years. This paper is aimed at exploring the early peripheral blood biomarkers of sudden death and providing a new perspective for clinical diagnosis and forensic pathology identification by integrated bioinformatics analysis.

Methods

Two microarray expression profiling datasets (GSE113079 and GSE31568) were downloaded from the Gene Expression Omnibus (GEO) database, and we identified differentially expressed lncRNAs, miRNAs, and mRNAs in CAD. Gene Ontology (GO) and KEGG pathway analyses of DEmRNAs were executed. A protein-protein interaction (PPI) network was constructed, and hub genes were identified. Finally, we constructed a competitive endogenous RNA (ceRNA) regulation network among lncRNAs, miRNAs, and mRNAs. Also, the 5 miRNAs of the ceRNA network were verified by RT-PCR.

Results

In total, 86 DElncRNAs, 148 DEmiRNAs, and 294 DEmRNAs were dysregulated in CAD. We received 12 GO terms and 5 pathways of DEmRNAs. 31 nodes and 78 edges were revealed in the PPI network. The top 10 genes calculated by degree method were identified as hub genes. Moreover, there were a total of 26 DElncRNAs, 5 DEmiRNAs, and 13 DEmRNAs in the ceRNA regulation network. We validated the 5 miRNAs of the ceRNA network by RT-PCR, which were consistent with the results of the microarray.

Conclusions

In this paper, a CAD-specific ceRNA network was successfully constructed, contributing to the understanding of the relationship among lncRNAs, miRNAs, and mRNAs. We identified potential peripheral blood biomarkers in CAD and provided novel insights into the development and progress of CAD.

SUBMITTER: He J

PROVIDER: S-EPMC7822659 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Comprehensive Analysis of ceRNA Regulation Network Involved in the Development of Coronary Artery Disease.

He Jiabei J Li Xinglong X Zhang Yao Y Zhang Qingqing Q Li Lianhong L

BioMed research international 20210114

<h4>Background</h4>Coronary artery disease (CAD) is one of the most common causes of sudden death with high morbidity in recent years. This paper is aimed at exploring the early peripheral blood biomarkers of sudden death and providing a new perspective for clinical diagnosis and forensic pathology identification by integrated bioinformatics analysis.<h4>Methods</h4>Two microarray expression profiling datasets (GSE113079 and GSE31568) were downloaded from the Gene Expression Omnibus (GEO) databa ...[more]

PMID: 33511207

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Dataset Information

Comprehensive Analysis of ceRNA Regulation Network Involved in the Development of Coronary Artery Disease.

Background

Methods

Results

Conclusions

Publications

Comprehensive Analysis of ceRNA Regulation Network Involved in the Development of Coronary Artery Disease.

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