Lipid Profile of Activated Macrophages and Contribution of Group V Phospholipase A₂.

ABSTRACT: Macrophages activated by Interleukin (IL)-4 (M2) or LPS+ Interferon (IFN)? (M1) perform specific functions respectively in type 2 inflammation and killing of pathogens. Group V phospholipase A₂ (Pla2g5) is required for the development and functions of IL-4-activated macrophages and phagocytosis of pathogens. Pla2g5-generated bioactive lipids, including lysophospholipids (LysoPLs), fatty acids (FAs), and eicosanoids, have a role in many diseases. However, little is known about their production by differentially activated macrophages. We performed an unbiased mass-spectrometry analysis of phospholipids (PLs), LysoPLs, FAs, and eicosanoids produced by Wild Type (WT) and Pla2g5-null IL-4-activated bone marrow-derived macrophages (IL-4)BM-Macs (M2) and (LPS+IFN?)BM-Macs (M1). Phosphatidylcholine (PC) was preferentially metabolized in (LPS+IFN?)BM-Macs and Phosphatidylethanolamine (PE) in (IL-4)BM-Macs, with Pla2g5 contributing mostly to metabolization of selected PE molecules. While Pla2g5 produced palmitic acid (PA) in (LPS+IFN?)BM-Macs, the absence of Pla2g5 increased myristic acid (MA) in (IL-4)BM-Macs. Among eicosanoids, Prostaglandin E₂ (PGE₂) and prostaglandin D₂ (PGD₂) were significantly reduced in (IL-4)BM-Macs and (LPS+IFN?)BM-Macs lacking Pla2g5. Instead, the IL-4-induced increase in 20-carboxy arachidonic acid (20CooH AA) was dependent on Pla2g5, as was the production of 12-hydroxy-heptadecatrienoic acid (12-HHTrE) in (LPS+IFN?)BM-Macs. Thus, Pla2g5 contributes to PE metabolization, PGE₂ and PGD₂ production independently of the type of activation, while in (IL-4)BM-Macs, Pla2g5 regulates selective lipid pathways and likely novel functions.

SUBMITTER: Koganesawa M 

PROVIDER: S-EPMC7823364 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature