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Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell.


ABSTRACT: Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO). Additional polymers were also introduced to improve biocatalyst immobilization. The reported design offers three main advantages: (i) by using glucose as the substrate for the both anode and cathode, a more compact and robust design is enabled, (ii) the system operates under air-saturating conditions, with no need for gas purge, and (iii) the combination of carbon nanostructures and a multi-enzyme cascade maximizes the sensitivity of the biosensor. Our design allows the reliable detection of glucose in the range of 0.1-7.0 mM, which is perfectly suited for common biofluids and industrial food samples.

SUBMITTER: Chansaenpak K 

PROVIDER: S-EPMC7825672 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Development of a Sensitive Self-Powered Glucose Biosensor Based on an Enzymatic Biofuel Cell.

Chansaenpak Kantapat K   Kamkaew Anyanee A   Lisnund Sireerat S   Prachai Pannaporn P   Ratwirunkit Patipat P   Jingpho Thitichaya T   Blay Vincent V   Pinyou Piyanut P  

Biosensors 20210107 1


Biofuel cells allow for constructing sensors that leverage the specificity of enzymes without the need for an external power source. In this work, we design a self-powered glucose sensor based on a biofuel cell. The redox enzymes glucose dehydrogenase (NAD-GDH), glucose oxidase (GOx), and horseradish peroxidase (HRP) were immobilized as biocatalysts on the electrodes, which were previously engineered using carbon nanostructures, including multi-wall carbon nanotubes (MWCNTs) and reduced graphene  ...[more]

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