Dataset Information

Intracellular Presence of Helicobacter pylori and Its Virulence-Associated Genotypes within the Vaginal Yeast of Term Pregnant Women.

ABSTRACT:

Background

Helicobacter pylori transmission routes are not entirely elucidated. Since yeasts are postulated to transmit this pathogen, this study aimed to detect and genotype intracellular H. pylori harbored within vaginal yeast cells.

Methods

A questionnaire was used to determine risk factors of H. pylori infection. Samples were seeded on Sabouraud Dextrose Agar and horse blood-supplemented Columbia agar. Isolated yeasts were identified using and observed by optical microscopy searching for intra-yeast H. pylori. Total yeast DNA, from one random sample, was extracted to search for H. pylori virulence genes by PCR and bacterial identification by sequencing.

Results

43% of samples contained yeasts, mainly Candida albicans (91%). Microscopy detected bacteria such as bodies and anti-H. pylori antibodies binding particles in 50% of the isolated yeasts. Total DNA extracted showed that 50% of the isolated yeasts were positive for H. pylori 16S rDNA and the sequence showed 99.8% similarity with H. pylori. In total, 32% of H. pylori DNA positive samples were cagA+ vacAs1a vacAm1 dupA-. No relationship was observed between possible H. pylori infection risk factors and vaginal yeasts harboring this bacterium.

Conclusion

H. pylori having virulent genotypes were detected within vaginal yeasts constituting a risk for vertical transmission of this pathogen.

SUBMITTER: Sanchez-Alonzo K

PROVIDER: S-EPMC7827377 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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Publications

Intracellular Presence of <i>Helicobacter pylori</i> and Its Virulence-Associated Genotypes within the Vaginal Yeast of Term Pregnant Women.

Sánchez-Alonzo Kimberly K Matamala-Valdés Lillian L Parra-Sepúlveda Cristian C Bernasconi Humberto H Campos Víctor L VL Smith Carlos T CT Sáez Katia K García-Cancino Apolinaria A

Microorganisms 20210108 1

<h4>Background</h4> <i>Helicobacter pylori</i> transmission routes are not entirely elucidated. Since yeasts are postulated to transmit this pathogen, this study aimed to detect and genotype intracellular <i>H. pylori</i> harbored within vaginal yeast cells.<h4>Methods</h4>A questionnaire was used to determine risk factors of <i>H. pylori</i> infection. Samples were seeded on Sabouraud Dextrose Agar and horse blood-supplemented Columbia agar. Isolated yeasts were identified using and observed by ...[more]

PMID: 33430099

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