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Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration.

ABSTRACT: The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and in vitro fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization ability and embryonic development potential. For complete information on the use and execution of this protocol, please refer to Miao et al. (2020).

SUBMITTER: Miao Y

PROVIDER: S-EPMC7829339 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration.

Miao Yilong Y Chen Jingyue J Gao Qian Q Xiong Bo B

STAR protocols 20210122 1

The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and <i>in vitro</i> fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization abil ...[more]

PMID: 33532742

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Nicotinamide mononucleotide supplementation reverses the declining quality of maternally aged oocytes

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