Glucocorticoid agonists enhance retinal stem cell self-renewal and proliferation.

ABSTRACT:

Background

Adult mammalian retinal stem cells (RSCs) readily proliferate, self-renew, and generate progeny that differentiate into all retinal cell types in vitro. RSC-derived progeny can be induced to differentiate into photoreceptors, making them a potential source for retinal cell transplant therapies. Despite their proliferative propensity in vitro, RSCs in the adult mammalian eye do not proliferate and do not have a regenerative response to injury. Thus, identifying and modulating the mechanisms that regulate RSC proliferation may enhance the capacity to produce RSC-derived progeny in vitro and enable RSC activation in vivo.

Methods

Here, we used medium-throughput screening to identify small molecules that can expand the number of RSCs and their progeny in culture. In v

SUBMITTER: Grise KN 

PROVIDER: S-EPMC7831262 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature