Dataset Information

Sequencing and characterization of the Megachile strupigera (Hymenoptera: Megachilidae) mitochondrial genome.

ABSTRACT: The mitochondrial genome (mitogenome) of Megachile strupigera (Hymenoptera: Megachilidae: Megachilinae) was determined in our study. The sequenced region is 15,193?bp with an A?+?T content of 83.44%, including 13 protein-coding genes, two ribosomal RNAs and 19 transfer RNAs. All PCGs are initiated by typical ATN codons and stop with the complete termination codon TAA, except for nad3 gene, which has an incomplete stop codon T. Bayesian method supported the monophyly of both Megachilidae and Apoidea. And within the Apoidea, Apidae and Megachilidae formed a sister clade to Colletidae.

SUBMITTER: Huang D

PROVIDER: S-EPMC7831656 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Sequencing and characterization of the Megachile strupigera (Hymenoptera: Megachilidae) mitochondrial genome.

Huang Dunyuan D Su Tianjuan T He Bo B Gu Ping P Liang Ai-Ping AP Zhu Chaodong C

Mitochondrial DNA. Part B, Resources 20160412 1

The mitochondrial genome (mitogenome) of Megachile strupigera (Hymenoptera: Megachilidae: Megachilinae) was determined in our study. The sequenced region is 15,193 bp with an A + T content of 83.44%, including 13 protein-coding genes, two ribosomal RNAs and 19 transfer RNAs. All PCGs are initiated by typical ATN codons and stop with the complete termination codon TAA, except for nad3 gene, which has an incomplete stop codon T. Bayesian method supported the monophyly of both Megachi ...[more]

PMID: 33537412

Similar Datasets

Project description:BackgroundOdorant-binding proteins (OBPs) are essential in insect's daily behaviors mediated by olfactory perception. Megachile saussurei Radoszkowski (Hymenoptera, Megachilidae) is a principal insect pollinating alfalfa (Medicago sativa) in Northwestern China. The olfactory function have been less conducted, which provides a lot of possibilities for our research.ResultsOur results showed that 20 OBPs were identified in total. Multiple sequence alignment analysis indicated MsauOBPs were highly conserved with a 6-cysteine motif pattern and all belonged to the classic subfamily, coding 113-196 amino acids and sharing 41.32%-99.12% amino acid identity with known OBPs of other bees. Phylogenetic analysis indicated there were certain homologies existed among MsauOBPs and most sequences were clustered with that of Osmia cornuta (Hymenoptera, Megachilidae). Expression analysis showed the identified OBPs were mostly enriched in antennae instead of other four body parts, especially the MsauOBP2, MsauOBP3, MsauOBP4, MsauOBP8, MsauOBP11 and MsauOBP17, in which the MsauOBP2, MsauOBP4 and MsauOBP8 presented obvious tissue-biased expression pattern. Molecular docking results indicated MsauOBP4 might be the most significant protein in recognizing alfalfa flower volatile 3-Octanone, while MsauOBP13 might be the most crucial protein identifying (Z)-3-hexenyl acetate. It was also found the lysine was a momentous hydrophilic amino acid in docking simulations.ConclusionIn this study, we identified and analyzed 20 OBPs of M. saussurei. The certain homology existed among these OBPs, while some degree of divergence could also be noticed, indicating the complex functions that different MsauOBPs performed. Besides, the M. saussurei and Osmia cornuta were very likely to share similar physiological functions as most of their OBPs were clustered together. MsauOBP4 might be the key protein in recognizing 3-Octanone, while MsauOBP13 might be the key protein in binding (Z)-3-hexenyl acetate. These two proteins might contribute to the alfalfa-locating during the pollination process. The relevant results may help determine the highly specific and effective attractants for M. saussurei in alfalfa pollination and reveal the molecular mechanism of odor-evoked pollinating behavior between these two species.

Dataset Information

Sequencing and characterization of the Megachile strupigera (Hymenoptera: Megachilidae) mitochondrial genome.

Publications

Sequencing and characterization of the <i>Megachile strupigera</i> (Hymenoptera: Megachilidae) mitochondrial genome.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets