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Gut CD4⁺ T cell phenotypes are a continuum molded by microbes, not by T_H archetypes.

ABSTRACT: CD4⁺ effector lymphocytes (T_eff) are traditionally classified by the cytokines they produce. To determine the states that T_eff cells actually adopt in frontline tissues in vivo, we applied single-cell transcriptome and chromatin analyses to colonic T_eff cells in germ-free or conventional mice or in mice after challenge with a range of phenotypically biasing microbes. Unexpected subsets were marked by the expression of the interferon (IFN) signature or myeloid-specific transcripts, but transcriptome or chromatin structure could not resolve discrete clusters fitting classic helper T cell (T_H) subsets. At baseline or at different times of infection, transcripts encoding cytokines or proteins commonly used as T_H markers were distributed in a polarized continuum, which was functionally validated. Clones derived from single progenitors gave rise to both IFN-γ- and interleukin (IL)-17-producing cells. Most of the transcriptional variance was tied to the infecting agent, independent of the cytokines produced, and chromatin variance primarily reflected activities of activator protein (AP)-1 and IFN-regulatory factor (IRF) transcription factor (TF) families, not the canonical subset master regulators T-bet, GATA3 or RORγ.

SUBMITTER: Kiner E

PROVIDER: S-EPMC7839314 | biostudies-literature |

REPOSITORIES: biostudies-literature

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