Effective reversal of hyperhydricity leading to efficient micropropagation of Dianthus chinensis L.

ABSTRACT: The effective reversion of hyperhydricity (HH) in Dianthus chinensis L. facilitated efficient in vitro production of hyperhydricity-free plantlets. Under routine sub-culture practice, the problem of HH arises after third sub-culture in agar (0.85%) gelled Murashige and Skoog (MS) medium containing 2.5 µM 6-benzyladenine (BA). To confirm the role of ethylene on hyperhydricity induction, an ethylene releasing compound ethephon (5 µM) was used in combination with 2.5 µM BA and demonstrated 100% HH with reduced stomatal aperture. Supplementation of 10 µM silver nitrate (AgNO₃) to 2.5 µM BA containing medium resulted HH reversion with reduced shoot number (19.0); however, addition of 5 µM cobalt chloride (CoCl₂) produced highest microshoots (202.0). The combination

SUBMITTER: Sreelekshmi R 

PROVIDER: S-EPMC7840827 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature