Dataset Information

Peripheral Inflammatory Cytokines and Lymphocyte Subset Features of Deceased COVID-19 Patients.

ABSTRACT:

Objective

To compare the difference of inflammatory cytokines and lymphocyte subsets between deceased patients and survivors with COVID-19.

Methods

This retrospective study included 254 confirmed patients from 10 January to 11 March, 2020, at Tongji Hospital of Wuhan, China. Laboratory and immunologic features were collected and analyzed, and the main outcomes focused on inflammatory cytokines and lymphocyte subsets.

Results

A trend of markedly higher levels of inflammatory cytokines as well as lower lymphocyte subset levels in deceased patients was observed compared with survivors. ROC curve analyses indicated that inflammatory cytokines and the decrease levels of T cell, Th (helper T cells) cell, Ts (suppressor T cells) cell, B cell, and NK cell along with Th/Ts ratio increase could be used to predict the death of COVID-19. Multivariate analyses showed that higher levels of IL-6, IL-8, and IL-10 remained significantly correlated with shorter survival time and that the amount of Ts cells was negatively associated with the possibility of death in COVID-19 patients. In conclusion, SARS-CoV-2 would cause lymphopenia and result in decreased lymphocyte subset cells, particularly in Ts cell counts, which further induces hyperinflammatory response and cytokine storm. IL-6, IL-8, IL-10, and Ts cell might be independent predictors for the poor outcome of COVID-19.

SUBMITTER: Jiang N

PROVIDER: S-EPMC7841449 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

Peripheral Inflammatory Cytokines and Lymphocyte Subset Features of Deceased COVID-19 Patients.

Jiang Nan N Li Zhijun Z Yang Bo B Jin Mengdi M Sun Yaoyao Y He Yang Y Liu Yang Y Wang Yueying Y Si Daoyuan D Ma Piyong P Zhang Jinnan J Liu Tianji T Yu Qiong Q

BioMed research international 20210122

<h4>Objective</h4>To compare the difference of inflammatory cytokines and lymphocyte subsets between deceased patients and survivors with COVID-19.<h4>Methods</h4>This retrospective study included 254 confirmed patients from 10 January to 11 March, 2020, at Tongji Hospital of Wuhan, China. Laboratory and immunologic features were collected and analyzed, and the main outcomes focused on inflammatory cytokines and lymphocyte subsets.<h4>Results</h4>A trend of markedly higher levels of inflammatory ...[more]

PMID: 33542929

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Project description:BackgroundCoronavirus disease 2019 (COVID-19) is a respiratory disorder caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which had rapidly spread all over the world and caused public health emergencies in the past two years. Although the diagnosis and treatment for COVID-19 have been well defined, the immune cell characteristics and the key lymphocytes subset alterations in COVID-19 patients have not been thoroughly investigated.MethodsThe levels of immune cells including T cells, B cells, and natural killer (NK) cells in 548 hospitalized COVID-19 patients, and 30 types of lymphocyte subsets in 125 hospitalized COVID-19 patients admitted to Wuhan Huoshenshan Hospital of China were measured using flow cytometry. The relationship between lymphocytes subsets with the cytokine interleukin-6 (IL-6) and the characteristics of lymphocyte subsets in single-cell RNA sequencing (scRNA-seq) data obtained from peripheral blood mononuclear cells (PBMCs) were also analysed in COVID-19 patients.ResultsIn this study, we found that patients with critical COVID-19 infection exhibited an overall decline in lymphocytes including CD4+ T cells, CD8+ T cells, total T cells, B cells, and NK cells compared to mild and severe patients. However, the number of lymphocyte subsets, such as CD21low CD38low B cells, effector T4 cells, and PD1+ depleted T8 cells, was moderately increased in critical COVID-19 patients compared to mild cases. Notably, except for effector memory T4 cells, plasma blasts and Tregs, the number of all lymphocyte subsets was markedly decreased in COVID-19 patients with IL-6 levels over 30-fold higher than those in healthy cases. Moreover, scRNA-seq data showed obvious differences in the distribution and numbers of lymphocyte subsets between COVID-19 patients and healthy persons, and subsets-specific marker genes of lymphocyte subsets including CD4, CD19, CCR7, and IL7R, were markedly decreased in COVID-19 patients compared with those in healthy cases.ConclusionA comprehensive decrease in immune cell and lymphocyte subsets in critical COVID-19 patients, and peripheral lymphocyte subset alterations showed a clear association with clinical characteristics.

Dataset Information

Peripheral Inflammatory Cytokines and Lymphocyte Subset Features of Deceased COVID-19 Patients.

Objective

Methods

Results

Publications

Peripheral Inflammatory Cytokines and Lymphocyte Subset Features of Deceased COVID-19 Patients.

Similar Datasets

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