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Potential Protective Activities of Extracts of Phellinus linteus and the Altered Expressions of GSTM3 on Age-Related Cataract.

ABSTRACT: Age-related cataract (ARC) is one of the leading causes of visual impairment and blindness worldwide among the elderly. Here, we used sodium selenite-induced cataract mouse model, which shares with similarities with human senile cataract to investigate whether the extracts of Phellinus linteus (PLE) could have the potential protective effects of ARC or not. The mice pups were randomly divided into 4 treatment groups (n?=?7): (1) normal saline on postpartum day 26; (2) Na selenite injected s.c on day 26; (3) Na selenite s.c on day 26+?gavaged PLE (40?mg/kg) on days 26-47; and (4) Na selenite s.c on day 26?+?resveratrol on days 26-47. On day 47, encapsulated lenses and plasma were analyzed for the levels of glutathione (GSH), superoxide dismutase (SOD), and malondialdehyde (MDA), a marker of lipid peroxidation. Lens epithelial cells (LECs) were also analyzed for the mRNA and protein expressions of glutathione S-transferase Mu (GSTM3). We demonstrated that PLE could prevent selenite-induced oxidative stress and cataract formation in mice by higher GSH and SOD and lower MDA in LECs, plasma, and liver tissues and the increases in the mRNA and protein expressions of GSTM3 in LECs. Our data show the increasing oxidative stress in selenite-induced cataract mice. Our data reveal the benefits of PLE for preventive activity in selenite-induced cataract in mice and there is a good possibility that PLE could ameliorate human senile cataract.

SUBMITTER: Lin CH

PROVIDER: S-EPMC7843177 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Potential Protective Activities of Extracts of Phellinus linteus and the Altered Expressions of GSTM3 on Age-Related Cataract.

Lin Cheng-Hsiu CH Shih Chun-Ching CC

Evidence-based complementary and alternative medicine : eCAM 20210121

Age-related cataract (ARC) is one of the leading causes of visual impairment and blindness worldwide among the elderly. Here, we used sodium selenite-induced cataract mouse model, which shares with similarities with human senile cataract to investigate whether the extracts of Phellinus linteus (PLE) could have the potential protective effects of ARC or not. The mice pups were randomly divided into 4 treatment groups (n = 7): (1) normal saline on postpartum day 26; (2) Na selenite i ...[more]

PMID: 33542742

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Project description:BackgroundComplementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. Phellinus linteus (P. linteus) as one of the CAMs has been used to modulate cancers, inflammation and allergic activities. However, little evidence has been shown about its underlying mechanism of action by which it exerts a beneficial role in dermatological disease in vivo. In this study, we examined the immunomodulatory effects of P. linteus on experimental atopic dermatitis (AD) and elucidated its action mechanism.MethodsThe immunomodulatory effect of total extract of P. linteus on IgE production by human myeloma U266B1 cells was measured by ELISA. To further identify the effective components, P. linteus was fractionated into methanol soluble, water soluble and boiling water soluble extracts. Each extract was treated to U266B1 cells and primary B cells to compare their inhibitory effects on IgE secretion. To test the in vivo efficacy, experimental atopic dermatitis (AD) was established by alternative treatment of DNCB and house dust mite extract into BALB/c mice. Water soluble extract of P. linteus (WA) or ceramide as a positive control were topically applied to ears of atopic mouse every day for 2 weeks and progression of the disease was estimated by the following criteria: (a) ear thickness, clinical score, (b) serum total IgE, IgG and mite specific IgE level by ELSIA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total ear cells and CD4(+) T cells by real time PCR and ELSIA.ResultsTreatment of total extracts of P. linteus to U266B1 inhibited IgE secretion. Among the diverse extracts of P. linteus, water soluble extract of P. linteus (WA) significantly reduced the IgE production in primary B cells and B cell line U266B1. Moreover, treatment of WA reduced AD symptoms such as ear swelling, erythema, and dryness and decreased recruitment of lymphocyte into the inflamed site. Interestingly WA treatment significantly reduced IgE level without affecting IgG levels and also down-regulated the levels of pathogenic cytokines (IL-4, IL-13, IL-12 and IFN-?) and chemokines (CCL17 and CCL22) involved in AD development.ConclusionsOur study indicates that protective effect of water soluble extract of P. linteus in atopic dermatitis is mediated by inhibiting IgE production and expression of AD associated pathogenic cytokines as well as chemokines, suggesting the beneficial effect of P. linteus to modulate allergic skin disease.

Dataset Information

Potential Protective Activities of Extracts of Phellinus linteus and the Altered Expressions of GSTM3 on Age-Related Cataract.

Publications

Potential Protective Activities of Extracts of <i>Phellinus linteus</i> and the Altered Expressions of <i>GSTM3</i> on Age-Related Cataract.

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