Unknown

Dataset Information

0

Presence and Characterization of a Novel cfr-Carrying Tn558 Transposon Derivative in Staphylococcus delphini Isolated From Retail Food.


ABSTRACT: Antimicrobial resistance has become a major public health threat. Food-related Staphylococcus species have received much attention due to their multidrug resistance. The cfr gene associated with multidrug resistance has been consistently detected in food-derived Staphylococcus species. In this retrospective study, we examined the prevalence of cfr-positive Staphylococcus strains isolated from poultry meat in different geographical areas of China from 2011 to 2016. Two cfr-positive Staphylococcus delphini strains were identified from poultry meat in China. Comparative and whole-genome analyses were performed to characterize the genetic features and overall antimicrobial resistance genes in the two S. delphini isolates 245-1 and 2794-1. Whole-genome sequencing showed that they both harbored a novel 20,258-bp cfr-carrying Tn558 transposon derivative on their chromosomes. The Tn558 derivative harbors multiple antimicrobial resistance genes, including the transferable multiresistance gene cfr, chloramphenicol resistance gene fexA, aminoglycoside resistance genes aacA-aphD and aadD, and bleomycin resistance gene ble. Surprisingly, within the Tn558 derivative, an active unconventional circularizable structure containing various resistance genes and a copy of a direct repeat sequence was identified by two-step PCR. Furthermore, core genome phylogenetic analysis revealed that the cfr-positive S. delphini strains were most closely related to S. delphini 14S03313-1 isolated from Japan in 2017 and 14S03319-1 isolated from Switzerland in 2017. This study is the first report of S. delphini harboring a novel cfr-carrying Tn558 derivative isolated from retail food. This finding raises further concerns regarding the potential threat to food safety and public health safety. The occurrence and dissemination of similar cfr-carrying transposons from diverse Staphylococcus species need further surveillance.

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7843796 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Presence and Characterization of a Novel <i>cfr</i>-Carrying Tn<i>558</i> Transposon Derivative in <i>Staphylococcus delphini</i> Isolated From Retail Food.

Zhang Feng F   Wu Shi S   Huang Jiahui J   Yang Runshi R   Zhang Jumei J   Lei Tao T   Dai Jingsha J   Ding Yu Y   Xue Liang L   Wang Juan J   Chen Moutong M   Wu Qingping Q  

Frontiers in microbiology 20210115


Antimicrobial resistance has become a major public health threat. Food-related <i>Staphylococcus</i> species have received much attention due to their multidrug resistance. The <i>cfr</i> gene associated with multidrug resistance has been consistently detected in food-derived <i>Staphylococcus</i> species. In this retrospective study, we examined the prevalence of <i>cfr</i>-positive <i>Staphylococcus</i> strains isolated from poultry meat in different geographical areas of China from 2011 to 20  ...[more]

Similar Datasets

| S-EPMC4059476 | biostudies-literature
| S-EPMC10874063 | biostudies-literature
| S-EPMC1797725 | biostudies-literature
| S-EPMC3697371 | biostudies-literature
| PRJNA82759 | ENA
| S-EPMC4432169 | biostudies-literature
| S-EPMC6209644 | biostudies-literature
| S-EPMC7171235 | biostudies-literature
| S-EPMC5899673 | biostudies-literature
| S-EPMC7074773 | biostudies-literature