Dataset Information

Escherichia coli-associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling.

ABSTRACT:

Background

Eradication of intramucosal Escherichia coli correlates with remission of periodic acid-Schiff-positive E coli-associated granulomatous colitis (GC). Treatment failures attributed to multidrug resistant (MDR) bacteria necessitate alternative approaches.

Hypothesis/objectives

Determine clinical outcome of E coli-associated GC in dogs treated based on antimicrobial susceptibility profiling and characterize E coli phylogeny and resistance mechanisms.

Animals

Twenty Boxers and 4 French Bulldogs with E coli-associated GC.

Methods

Culture, antimicrobial susceptibility profiling, and molecular characterization of E coli were performed and response to treatment was evaluated.

Results

Initial biopsy sample culture yielded fluoroquinolone-sensitive (FQ-S) E coli from 9/24 dogs and fluoroquinolone-resistant (FQ-R) E coli from 15/24. All but 1 FQ-R E coli were MDR with susceptibility to macrophage-penetrating antimicrobials restricted to carbapenems in 13/15 dogs. Of 22/24 treated based on susceptibility profiling, 8/9 FQ-S dogs had complete initial clinical response (CR) during fluoroquinolone (FQ) treatment, whereas 9/13 FQ-R dogs had complete or partial response (PR) during meropenem or doxycycline treatment. In 5/9 FQ-S and 12/13 FQ-R dogs with follow-up ?3 months, CR was sustained in 5/5 FQ-S (median, 25?months; range, 4-46) whereas 6/12 FQ-R had long-term CR (median, 59?months; range 15-102), 4/12 PR (median, 19?months; range, 5-65), and 2/12 had no response (NR). Four dogs with long-term follow-up died within 4?years of diagnosis, including 2 euthanized for refractory colitis. Escherichia coli were genetically diverse. Fluoroquinolone resistance was associated with mutations in gyrA and parC, with plasmid-mediated resistance less common.

Conclusions and clinical importance

Antimicrobial treatment guided by susceptibility profiling was associated with positive long-term outcomes in >80% of cases. Fluoroquinolone-resistance was widespread and not clonal. Further study is required to optimize treatment for dogs with MDR E coli-associated GC.

SUBMITTER: Manchester AC

PROVIDER: S-EPMC7848323 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Escherichia coli-associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling.

Manchester Alison C AC Dogan Belgin B Guo Yongli Y Simpson Kenneth W KW

Journal of veterinary internal medicine 20201215 1

<h4>Background</h4>Eradication of intramucosal Escherichia coli correlates with remission of periodic acid-Schiff-positive E coli-associated granulomatous colitis (GC). Treatment failures attributed to multidrug resistant (MDR) bacteria necessitate alternative approaches.<h4>Hypothesis/objectives</h4>Determine clinical outcome of E coli-associated GC in dogs treated based on antimicrobial susceptibility profiling and characterize E coli phylogeny and resistance mechanisms.<h4>Animals</h4>Twenty ...[more]

PMID: 33321554

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Dataset Information

Escherichia coli-associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling.

Background

Hypothesis/objectives

Animals

Methods

Results

Conclusions and clinical importance

Publications

Escherichia coli-associated granulomatous colitis in dogs treated according to antimicrobial susceptibility profiling.

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