Dataset Information

High serum creatinine concentration is associated with metabolic perturbations in dogs.

ABSTRACT:

Background

The kidneys have many essential metabolic functions, and metabolic disturbances during decreased renal function have not been studied extensively.

Objectives

To identify metabolic changes in blood samples with increased serum creatinine concentration, indicating decreased glomerular filtration.

Animals

Clinical samples analyzed using a nuclear magnetic resonance (NMR) based metabolomics platform. The case group consisted of 23 samples with serum creatinine concentration?>125??mol/L, and the control group of 873 samples with serum creatinine concentration within the reference interval.

Methods

Biomarker association with increased serum creatinine concentration was evaluated utilizing 3 statistical approaches: Wilcoxon rank-sum test, logistic regression analysis (false discovery rate (FDR)-corrected P-values), and random forest classification. Medians of the biomarkers were compared to reference intervals. A heatmap and box plots were used to represent the differences.

Results

All 3 statistical approaches identified similar analytes associated with increased serum creatinine concentrations. The percentages of citrate, tyrosine, branched-chain amino acids, valine, leucine, albumin, linoleic acid and the ratio of phenylalanine to tyrosine differed significantly using all statistical approaches, acetate differed using the Wilcoxon test and random forest, docosapentaenoic acid percentage only using logistic regression (P Conclusions and clinical importanceWe identified several metabolic changes associated with increased serum creatinine concentrations, including prospective diagnostic markers and therapeutic targets. Further research is needed to verify the association of these changes with the clinical state of the dog. The NMR metabolomics test is a promising tool for improving diagnostic testing and management of renal diseases in dogs.

SUBMITTER: Ottka C

PROVIDER: S-EPMC7848334 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Ottka Claudia C Vapalahti Katariina K Määttä Ann-Marie AM Huuskonen Nanna N Sarpanen Sinikka S Jalkanen Liisa L Lohi Hannes H

Journal of veterinary internal medicine 20201221 1

<h4>Background</h4>The kidneys have many essential metabolic functions, and metabolic disturbances during decreased renal function have not been studied extensively.<h4>Objectives</h4>To identify metabolic changes in blood samples with increased serum creatinine concentration, indicating decreased glomerular filtration.<h4>Animals</h4>Clinical samples analyzed using a nuclear magnetic resonance (NMR) based metabolomics platform. The case group consisted of 23 samples with serum creatinine concen ...[more]

PMID: 33349961

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Project description:A prospective study was conducted in client-owned geriatric dogs to evaluate the short-term effects of a test food on serum symmetric dimethylarginine (SDMA) and creatinine (Cr) concentrations. Test food contained functional lipids (fish oil), antioxidants (lipoic acid, vitamins C and E), L-carnitine, botanicals (fruits and vegetables), controlled sodium concentration, and high quality protein sources (high bioavailability and an ideal amino acid composition). Dogs (n = 210) were fed either test food or owner's-choice foods (non-nutritionally controlled cohort). Dogs were included based on age and body weight: small (6.8 to 11.4 kg) and medium dogs (11.5 to 22.7 kg) were ≥ 9 years, whereas dogs >22.7 kg were ≥ 7 years at baseline. At baseline, all dogs had to have serum Cr concentrations within the reference interval and be free of chronic disease. Renal function biomarkers and urinalysis results at baseline, and after consuming test food or owner's-choice foods for 3 and 6 months, were evaluated. Only dogs consuming test food showed significant decreases in serum SDMA and Cr concentrations (both P ≤ 0.05) across time. At baseline or during the 6-month feeding trial, 18 dogs (8.6%) had increased serum SDMA, but normal serum Cr, consistent with IRIS Stage 1 chronic kidney disease. This included 9 dogs fed test food and 9 dogs fed owner's-choice foods. Compared with baseline, after feeding 9 dogs test food for 6 months, serum SDMA decreased in 8 dogs and increased in 1 dog. After feeding 9 dogs owner's-choice foods for 6 months, serum SDMA decreased in 4 dogs and increased in 4 dogs (remained stable in 1 dog). The decreases in serum SDMA and Cr concentrations were significant (both P = 0.03) only for dogs fed test food. These results suggest that nonazotemic dogs with elevated serum SDMA (early renal insufficiency) when fed a test food designed to promote healthy aging are more likely to demonstrate improved renal function compared with dogs fed owner's-choice foods.

Dataset Information

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Background

Objectives

Animals

Methods

Results

Publications

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Similar Datasets

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