Dataset Information

High serum creatinine concentration is associated with metabolic perturbations in dogs.

ABSTRACT:

Background

The kidneys have many essential metabolic functions, and metabolic disturbances during decreased renal function have not been studied extensively.

Objectives

To identify metabolic changes in blood samples with increased serum creatinine concentration, indicating decreased glomerular filtration.

Animals

Clinical samples analyzed using a nuclear magnetic resonance (NMR) based metabolomics platform. The case group consisted of 23 samples with serum creatinine concentration?>125??mol/L, and the control group of 873 samples with serum creatinine concentration within the reference interval.

Methods

Biomarker association with increased serum creatinine concentration was evaluated utilizing 3 statistical approaches: Wilcoxon rank-sum test, logistic regression analysis (false discovery rate (FDR)-corrected P-values), and random forest classification. Medians of the biomarkers were compared to reference intervals. A heatmap and box plots were used to represent the differences.

Results

All 3 statistical approaches identified similar analytes associated with increased serum creatinine concentrations. The percentages of citrate, tyrosine, branched-chain amino acids, valine, leucine, albumin, linoleic acid and the ratio of phenylalanine to tyrosine differed significantly using all statistical approaches, acetate differed using the Wilcoxon test and random forest, docosapentaenoic acid percentage only using logistic regression (P Conclusions and clinical importanceWe identified several metabolic changes associated with increased serum creatinine concentrations, including prospective diagnostic markers and therapeutic targets. Further research is needed to verify the association of these changes with the clinical state of the dog. The NMR metabolomics test is a promising tool for improving diagnostic testing and management of renal diseases in dogs.

SUBMITTER: Ottka C

PROVIDER: S-EPMC7848334 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Publications

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Ottka Claudia C Vapalahti Katariina K Määttä Ann-Marie AM Huuskonen Nanna N Sarpanen Sinikka S Jalkanen Liisa L Lohi Hannes H

Journal of veterinary internal medicine 20201221 1

<h4>Background</h4>The kidneys have many essential metabolic functions, and metabolic disturbances during decreased renal function have not been studied extensively.<h4>Objectives</h4>To identify metabolic changes in blood samples with increased serum creatinine concentration, indicating decreased glomerular filtration.<h4>Animals</h4>Clinical samples analyzed using a nuclear magnetic resonance (NMR) based metabolomics platform. The case group consisted of 23 samples with serum creatinine concen ...[more]

PMID: 33349961

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Project description:GoalBody mass index (BMI) in early pregnancy is a critical risk factor for hypertensive disorders of pregnancy (HDP). The pathobiology of the interplay between BMI and HDP is not fully understood and represents the focus of this investigation.MethodsBMI and 1st-trimester serum samples were obtained from the Global Alliance to Prevent Prematurity and Stillbirth repository for 154 women (105 without HDP and 49 with HDP). Metabotyping was conducted using ultra-high-performance liquid-chromatography high-resolution mass spectrometry (UHPLC HR-MS). Multivariable linear regression and logistic models were used to determine metabolites and pathway perturbations associated with BMI in women with and without HDP, and to determine metabolites and pathway perturbations associated with HDP for women in categories of obese, overweight, and normal weight based on the 1st trimester BMI. These outcome-associated signals were identified or annotated by matching against an in-house physical standards library and public database. Pathway analysis was conducted by the Mummichog algorithm in MetaboAnalyst.ResultVitamin D3 and lysine metabolism were enriched to associate with BMI for women with and without HDP. Tryptophan metabolism enrichment was associated with HDP in all the BMI categories. Pregnant women who developed HDP showed more metabolic perturbations with BMI (continuous) than those without HDP in their 1st-trimester serum. The HDP-associated pathways for women with normal weight indicated inflammation and immune responses. In contrast, the HDP-associated pathways for women of overweight and obese BMI indicated metabolic syndromes with disorders in glucose, protein, and amino acid, lipid and bile acid metabolism, and oxidative and inflammatory stress.ConclusionHigh first-trimester BMI indicates underlying metabolic syndromes, which play critical roles in HDP development. Vitamin D3 and tryptophan metabolism may be the targets to guide nutritional interventions to mitigate metabolic and inflammatory stress in pregnancy and reduce the onset of HDP.

Dataset Information

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Background

Objectives

Animals

Methods

Results

Publications

High serum creatinine concentration is associated with metabolic perturbations in dogs.

Similar Datasets

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