Dataset Information
The mutational landscape of spinal chordomas and their sensitive detection using circulating tumor DNA.
Ontology highlight
ABSTRACT: Background
Chordomas are the most common primary spinal column malignancy in the United States. The aim of this study was to determine whether chordomas may be detected by evaluating mutations in circulating tumor DNA (ctDNA).Methods
Thirty-two patients with a biopsy-confirmed diagnosis of chordoma had blood drawn pre-operatively and/or at follow-up appointments. Mutations in the primary tumor were identified by whole exome sequencing and liquid biopsy by ddPCR and/or RACE-Seq was used to detect one or more of these mutations in plasma ctDNA at concurrent or later time points.Results
At the time of initial blood draw, 87.1% of patients were ctDNA positive (P <.001). Follow-up blood draws in twenty of the patients suggest that ctDNA levels may reflect the clinical status of the disease. Patients with positive ctDNA levels were more likely to have greater mutant allele frequencies in their primary tumors (P = .004) and undergo radiotherapy (P = .02), and the presence of ctDNA may correlate with response to systemic chemotherapy and/or disease recurrence.Conclusions
Detection of ctDNA mutations may allow for the detection and monitoring of disease progression for chordomas.
SUBMITTER: Mattox AK
PROVIDER: S-EPMC7850091 | biostudies-literature | 2021 Jan-Dec
REPOSITORIES: biostudies-literature
Publications
The mutational landscape of spinal chordomas and their sensitive detection using circulating tumor DNA.
Neuro-oncology advances 20201208 1
<h4>Background</h4>Chordomas are the most common primary spinal column malignancy in the United States. The aim of this study was to determine whether chordomas may be detected by evaluating mutations in circulating tumor DNA (ctDNA).<h4>Methods</h4>Thirty-two patients with a biopsy-confirmed diagnosis of chordoma had blood drawn pre-operatively and/or at follow-up appointments. Mutations in the primary tumor were identified by whole exome sequencing and liquid biopsy by ddPCR and/or RACE-Seq wa ...[more]