Ontology highlight
ABSTRACT:
SUBMITTER: Yammine A
PROVIDER: S-EPMC7854220 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Bio-protocol 20190605 11
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local environment polarity experienced by the tryptophan(s) upon the addition of a binding partner or ligand. The quenching can arise from local changes near the interaction site or from binding-induced conformational changes. In cases where the titrant absorb ...[more]