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Multiplex recording of cellular events over time on CRISPR biological tape.

ABSTRACT: Although dynamics underlie many biological processes, our ability to robustly and accurately profile time-varying biological signals and regulatory programs remains limited. Here we describe a framework for storing temporal biological information directly in the genomes of a cell population. We developed a "biological tape recorder" in which biological signals trigger intracellular DNA production that is then recorded by the CRISPR-Cas adaptation system. This approach enables stable recording over multiple days and accurate reconstruction of temporal and lineage information by sequencing CRISPR arrays. We further demonstrate a multiplexing strategy to simultaneously record the temporal availability of three metabolites (copper, trehalose, and fucose) in the environment of a cell population over time. This work enables the temporal measurement of dynamic cellular states and environmental changes and suggests new applications for chronicling biological events on a large scale.

SUBMITTER: Sheth RU

PROVIDER: S-EPMC7869111 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Multiplex recording of cellular events over time on CRISPR biological tape.

Sheth Ravi U RU Yim Sung Sun SS Wu Felix L FL Wang Harris H HH

Science (New York, N.Y.) 20171123 6369

Although dynamics underlie many biological processes, our ability to robustly and accurately profile time-varying biological signals and regulatory programs remains limited. Here we describe a framework for storing temporal biological information directly in the genomes of a cell population. We developed a "biological tape recorder" in which biological signals trigger intracellular DNA production that is then recorded by the CRISPR-Cas adaptation system. This approach enables stable recording ov ...[more]

PMID: 29170279

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