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CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.

ABSTRACT: Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time-consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.

SUBMITTER: Anderson DP

PROVIDER: S-EPMC7266498 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering.

Anderson Daniel Paolo DP Benns Henry James HJ Tate Edward William EW Child Matthew Andrew MA

Molecular systems biology 20200601 6

Rational molecular engineering of proteins with CRISPR-based approaches is challenged by the gene-centric nature of gRNA design tools. To address this, we have developed CRISPR-TAPE, a protein-centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology-directed repair for engineering purposes, removing time-consuming post hoc curation, simplifying gRNA outputs and reducing ...[more]

PMID: 32485092

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