Ontology highlight
ABSTRACT:
SUBMITTER: Selyutina A
PROVIDER: S-EPMC7871456 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Selyutina Anastasia A Persaud Mirjana M Lee Kyeongeun K KewalRamani Vineet V Diaz-Griffero Felipe F
Cell reports 20200901 13
HIV-1 reverse transcription (RT) occurs before or during uncoating, but the cellular compartment where RT and uncoating occurs is unknown. Using imaging and biochemical assays to track HIV-1 capsids in the nucleus during infection, we demonstrated that higher-order capsid complexes and/or complete cores containing the viral genome are imported into the nucleus. Inhibition of RT does not prevent capsid nuclear import; thus, RT may occur in nuclear compartments. Cytosolic and nuclear fractions of ...[more]