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The complete mitochondrial genome of huperzine A-producing endophytic fungus Penicillium polonicum.

ABSTRACT: Huperzine A-producing fungus Penicillium polonicum Hy4 (CCTCC No.M2010086) was isolated from Huperzia serrata (Thunb) Trev. The complete mitochondrial genome of P. polonicum is 28?192?bp in length, containing 15 protein-encoding genes, 27 tRNA genes and two rRNA genes. The whole mitogenome is high in AT content (74.40%) and low in GC content (25.60%). The mitochondrial gene order and arrangement of P. polonicum are identical to those of other Penicillium. Phylogenetic analysis based on 14 concatenated protein-encoding genes showed that P. polonicum was close to P. solitum. This study reports the complete mitogenome of P. polonicum for the first time and provides valuable information for further exploration of mitochondrial evolution.

SUBMITTER: Kang X

PROVIDER: S-EPMC7871816 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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The complete mitochondrial genome of huperzine A-producing endophytic fungus Penicillium polonicum.

Kang Xincong X Liu Chichuan C Liu Dongbo D Zeng Lu L Shi Qianqian Q Qian Kun K Xie Bingyan B

Mitochondrial DNA. Part B, Resources 20160411 1

Huperzine A-producing fungus Penicillium polonicum Hy4 (CCTCC No.M2010086) was isolated from Huperzia serrata (Thunb) Trev. The complete mitochondrial genome of P. polonicum is 28 192 bp in length, containing 15 protein-encoding genes, 27 tRNA genes and two rRNA genes. The whole mitogenome is high in AT content (74.40%) and low in GC content (25.60%). The mitochondrial gene order and arrangement of P. polonicum are identical to those of other Penicillium. Phylo ...[more]

PMID: 33644344

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Project description:Endophytic fungi are symbiotic with plants and possess multienzyme systems showing promising metabolite potency with region selectivity and stereoselectivity. The aim of this study was to use these special microorganisms as an in vitro model to mimic the potential mammalian metabolites of a natural iridoid gentiopicroside (GPS, compound 1). The fungi isolated from a medicinal plant, Dendrobium candidum Wall. ex Lindl., were screened for their biotransformation abilities with GPS as the substrate, and one strain with high converting potency was identified as Penicillium crustosum 2T01Y01 on the basis of the sequence of the internal transcribed spacer of the ribosomal DNA region. Upon the optimized incubation of P. crustosum 2T01Y01 with the substrate, seven deglycosylated metabolites were detected by ultraperformance liquid chromatography/quadrupole time of flight mass spectrometry (UPLC/Q-TOF MS). Preparative-scale biotransformation with whole cells of the endophytic fungus resulted in the production of five metabolites, including three novel ones, 5?-(hydroxymethyl)-6?-methyl-3,4,5,6-tetrahydropyrano[3,4-c]pyran-1(8H)-one (compound 2), (Z)-4-(1-hydroxybut-3-en-2-yl)-5,6-dihydropyran-2-one (compound 3), and (E)-4-(1-hydroxybut-3-en-2-yl)-5,6-dihydropyran-2-one (compound 4), along with two known ones, 5?-(hydroxymethyl)-6?-methyl-1H,3H-5,6-dihydropyrano[3,4-c]pyran-1(3H)-one (compound 5) and 5?-(hydroxymethyl)-6?-methyl-5,6-dihydropyrano[3,4-c]pyran-1(3H)-one (compound 6), aided by nuclear magnetic resonance and high-resolution mass spectral analyses. The other two metabolites were tentatively identified by online UPLC/Q-TOF MS as 5-hydroxymethyl-5,6-dihydroisochromen-1-one (compound 7) and 5-hydroxymethyl-3,4,5,6-tetrahydroisochromen-1-one (compound 8), and compound 8 is a new metabolite. To test the metabolic mechanism, the ?-glucosidase activity of the fungus P. crustosum 2T01Y01 was assayed with ?-nitrophenyl-?-d-glucopyranoside as a probe substrate, and the pathway of GPS biotransformation by strain 2T01Y01 is proposed. In addition, the hepatoprotective activities of GPS and metabolite compounds 2, 5, and 6 against human hepatocyte line HL-7702 injury induced by hydrogen peroxide were evaluated.

Dataset Information

The complete mitochondrial genome of huperzine A-producing endophytic fungus Penicillium polonicum.

Publications

The complete mitochondrial genome of huperzine A-producing endophytic fungus <i>Penicillium polonicum</i>.

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