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DNA Polymerase ? Interacts with Both the TRAF-like and UBL1-2 Domains of USP7.


ABSTRACT: Reversible protein ubiquitination is an essential signaling mechanism within eukaryotes. Deubiquitinating enzymes are critical to this process, as they mediate removal of ubiquitin from substrate proteins. Ubiquitin-specific protease 7 (USP7) is a prominent deubiquitinating enzyme, with an extensive network of interacting partners and established roles in cell cycle activation, immune responses and DNA replication. Characterized USP7 substrates primarily interact with one of two major binding sites outside the catalytic domain. These are located on the USP7 N-terminal TRAF-like (TRAF) domain and the first and second UBL domains (UBL1-2) within the C-terminal tail. Here, we report that DNA polymerase iota (Pol ?) is a novel USP7 substrate that interacts with both TRAF and UBL1-2. Through the use of biophysical approaches and mutational analysis, we characterize both interfaces and demonstrate that bipartite binding to both USP7 domains is required for efficient Pol ? deubiquitination. Together, these data establish a new bipartite mode of USP7 substrate binding.

SUBMITTER: Ashton NW 

PROVIDER: S-EPMC7873624 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

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DNA Polymerase ι Interacts with Both the TRAF-like and UBL1-2 Domains of USP7.

Ashton Nicholas W NW   Ashton Nicholas W NW   Valles Gabrielle J GJ   Jaiswal Nancy N   Bezsonova Irina I   Woodgate Roger R  

Journal of molecular biology 20201203 2


Reversible protein ubiquitination is an essential signaling mechanism within eukaryotes. Deubiquitinating enzymes are critical to this process, as they mediate removal of ubiquitin from substrate proteins. Ubiquitin-specific protease 7 (USP7) is a prominent deubiquitinating enzyme, with an extensive network of interacting partners and established roles in cell cycle activation, immune responses and DNA replication. Characterized USP7 substrates primarily interact with one of two major binding si  ...[more]

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