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Gastruloid Development Competence Discriminates Different States of Pluripotency.

ABSTRACT: Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ? 95%) to primed (GFE = 0) state. Specifically, we show that primed EpiSCs fail to generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually fail to develop into elongated gastruloids. Moreover, we characterized proline-induced cells (PiCs), a LIF-dependent reversible early-primed state of pluripotency, and show that PiCs are able to generate gastruloids (GFE ? 50%) and are also competent to differentiate into primordial germ cell-like cells. Thus, we propose the GFE assay as a valuable functional tool to discriminate different states of the pluripotency continuum.

SUBMITTER: Cermola F

PROVIDER: S-EPMC7878839 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Gastruloid Development Competence Discriminates Different States of Pluripotency.

Cermola Federica F D'Aniello Cristina C Tatè Rosarita R De Cesare Dario D Martinez-Arias Alfonso A Minchiotti Gabriella G Patriarca Eduardo Jorge EJ

Stem cell reports 20210121 2

Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ≥ 95%) to primed (GFE = 0) state. Specifically, we show that primed EpiSCs fail to generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually fail to develop into elongated gastruloids. More ...[more]

PMID: 33482102

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