Ontology highlight
ABSTRACT:
SUBMITTER: Sorge E
PROVIDER: S-EPMC7880479 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Sorge Eberhard E Demidov Dmitri D Lermontova Inna I Houben Andreas A Conrad Udo U
PloS one 20210212 2
Determining the function of proteins remains a key task of modern biology. Classical genetic approaches to knocking out protein function in plants still face limitations, such as the time-consuming nature of generating homozygous transgenic lines or the risk of non-viable loss-of-function phenotypes. We aimed to overcome these limitations by acting downstream of the protein level. Chimeric E3 ligases degrade proteins of interest in mammalian cell lines, Drosophila melanogaster embryos, and trans ...[more]