Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants.
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ABSTRACT:
SUBMITTER: Baudisch B
PROVIDER: S-EPMC5811635 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants.
Baudisch Bianca B Pfort Ingrid I Sorge Eberhard E Conrad Udo U
Frontiers in plant science 20180209
Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation <i>via</i> the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest <i>in planta</i> using specific, high-affinity binding proteins. ...[more]
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