Ontology highlight
ABSTRACT:
SUBMITTER: Maas RGC
PROVIDER: S-EPMC7881265 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
STAR protocols 20210209 1
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream as ...[more]