Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes.
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ABSTRACT:
SUBMITTER: Maas RGC
PROVIDER: S-EPMC7881265 | biostudies-literature | 2021 Mar
REPOSITORIES: biostudies-literature
Publications
Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes.
Maas Renee G C RGC Lee Soah S Harakalova Magdalena M Snijders Blok Christian J B CJB Goodyer William R WR Hjortnaes Jesper J Doevendans Pieter A F M PAFM Van Laake Linda W LW van der Velden Jolanda J Asselbergs Folkert W FW Wu Joseph C JC Sluijter Joost P G JPG Wu Sean M SM Buikema Jan W JW
STAR protocols 20210209 1
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). Here, we describe a cost-effective strategy for the subsequent massive expansion (>250-fold) of high-purity hiPSC-CMs relying on two aspects: removal of cell-cell contacts and small-molecule inhibition with CHIR99021. The protocol maintains CM functionality, allows cryopreservation, and the cells can be used in downstream as ...[more]
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