Dataset Information

Characterization of ??T cells in lung of Plasmodium yoelii-infected C57BL/6 mice.

ABSTRACT:

Background

Malaria has high morbidity and mortality rates in some parts of tropical and subtropical countries. Besides respiratory and metabolic function, lung plays a role in immune system. ??T cells have multiple functions in producing cytokines and chemokines, regulating the immune response by interacting with other cells. It remains unclear about the role of ??T cells in the lung of mice infected by malaria parasites.

Methods

Flow cytometry (FCM) was used to evaluate the frequency of ??T cells and the effects of ??T cells on the phenotype and function of B and T cells in Plasmodium yoelii-infected wild-type (WT) or ??TCR knockout (??T KO) mice. Haematoxylin-eosin (HE) staining was used to observe the pathological changes in the lungs.

Results

The percentage and absolute number of ??T cells in the lung increased after Plasmodium infection (p?+, IL-5⁺, IL-6⁺, IL-21⁺, IL-1?⁺, and IL-17⁺ ??T cells were increased (p?+ cells and absolute numbers of CD3⁺ cells, CD3⁺ CD4⁺ cells, CD3⁺ CD8⁺ cells decreased in ??T KO infected mice (p??0.05). While, the percentage of IFN-?-expressing CD3⁺ and CD3⁺ CD8⁺ cells increased in ??T KO infected mice (p?+, ICOS⁺, and CD80⁺ B cells between the WT infected and ??T KO infected mice (p?>?0.05).

Conclusions

The content, phenotype, and function of ??T cells in the lung of C57BL/6 mice were changed after Plasmodium infection. ??T cells contribute to T cell immune response in the progress of Plasmodium infection.

SUBMITTER: Wei H

PROVIDER: S-EPMC7885449 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Publications

Characterization of γδT cells in lung of Plasmodium yoelii-infected C57BL/6 mice.

Wei Haixia H Jin Chenxi C Peng Anping A Xie Hongyan H Xie Shihao S Feng Yuanfa Y Xie Anqi A Li Jiajie J Fang Chao C Yang Quan Q Qiu Huaina H Qi Yanwei Y Yin Zhinan Z Wang Xinhua X Huang Jun J

Malaria journal 20210215 1

<h4>Background</h4>Malaria has high morbidity and mortality rates in some parts of tropical and subtropical countries. Besides respiratory and metabolic function, lung plays a role in immune system. γδT cells have multiple functions in producing cytokines and chemokines, regulating the immune response by interacting with other cells. It remains unclear about the role of γδT cells in the lung of mice infected by malaria parasites.<h4>Methods</h4>Flow cytometry (FCM) was used to evaluate the frequ ...[more]

PMID: 33588839

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